Enzymatic hydrolysis of green tea [Camellia sinensis] seed extract and its activity on 5alpha-reductase inhibition

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-02, Vol.70 (2), p.387-394
• Main Authors: Park, J.S.(AMOREPACIFIC R and D Center (Korea R.)), Yeom, M.H, Park, W.S, Joo, K.M, Rho, H.S, Kim, D.H, Chang, I.S
• Format: Article
• Language: English
• Subjects: CAMELIA SINENSIS; CAMELLIA SINENSIS; Cell Line; Chromatography, High Pressure Liquid; Color; ENZYMATIC HYDROLYSIS; Enzyme Inhibitors - pharmacology; EXTRACTOS DE SEMILLAS; EXTRAIT DE GRAINES; Flavonoids - chemistry; Flavonoids - isolation & purification; Flavonoids - pharmacology; GLICOSIDOS; GLYCOSIDE; GLYCOSIDES; Glycosylation - drug effects; HIDROLISIS ENZIMATICA; Humans; HYDROLYSE ENZYMATIQUE; Hydrolysis; KAEMPFEROL; Molecular Structure; OXIDOREDUCTASES; Oxidoreductases - antagonists & inhibitors; Oxidoreductases - genetics; Oxidoreductases - metabolism; OXIDORREDUCTASAS; OXYDOREDUCTASE; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - metabolism; Plant Extracts - pharmacology; SEED EXTRACTS; Seeds - chemistry; Tea - chemistry
• ISSN: 0916-8451; 1347-6947

Two kaempferol glycosides were isolated from green tea seed extract (GTSE). After conducting a structure analysis, these two compounds were identified as kaempferol-3-0-[2-0-beta-D-galactopyranosyl-6-0-alpha-L-rhamnopyranosyl]-beta-D-glucopyranoside (compound 1) and kaempferol-3-0-[2-0-beta-D-xylopyranosyl-6-0-alpha-L-rhanmopyranosyl]-beta-D-glucopyranoside (compound 2). These two compounds were hydrolysed by o-glycolytic enzymes for the production of kaempferol. After performing several reactions, we found the optimum enzyme combination, a reaction with beta-galactosidase and hesperidinase. Finally, we produced kaempferol of above 95% Purity. The 5alpha-reductase inhibition activities of GTSE hydrolysate (GTSE-H) containing kaempferol were evaluated by the contact cell-based metabolic method using a stable HEK 293 cell line. GTSE-H showed a good inhibition effect on HEK 293 cell lines both type 1 and type 2 on 5alpha-reductase. Especially, GTSE-H inhibited type 2 with kaempferol content dependency. The results indicate that the inhibition activity of hydrolysate on 5alpha-reductase type 2 increases in accordance with kaempferol content.