Carbohydrate specificity of lectins from Boletopsis leucomelas and Aralia cordate

The carbohydrate specificity of three novel lectins, Boletopsis leucomelas lectin (BLL), Aralia cordate lectin (ACL), and Wasabia japonica lectin (WJL), was examined by frontal affinity chromatography using a panel of fluorescently labeled 47 oligosaccharides. The results indicate that BLL recognize...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-02, Vol.70 (2), p.542-545
Main Authors: Koyama, Y.(Shizuoka Univ. (Japan)), Suzuki, T, Odani, S, Nakamura, S, Kominami, J, Hirabayashi, J, Isemura, M
Format: Article
Language:English
Subjects:
ARALIA
Aralia - chemistry
Aralia cordate
Basidiomycota - chemistry
Biological and medical sciences
Boletopsis leucomelas
BRASSICACEAE
CARBOHIDRATOS
CARBOHYDRATES
Carbohydrates - chemistry
CHAMPIGNON COMESTIBLE
CHEMICOPHYSICAL PROPERTIES
CHROMATOGRAPHIE
CHROMATOGRAPHY
CROMATOGRAFIA
EDIBLE FUNGI
frontal affinity chromatography
Fundamental and applied biological sciences. Psychology
GLUCIDE
lectin
LECTINAS
LECTINE
LECTINS
Lectins - chemistry
Lectins - metabolism
Molecular Conformation
PROPIEDADES FISICOQUIMICAS
PROPRIETE PHYSICOCHIMIQUE
SETAS COMESTIBLES
Substrate Specificity
Wasabia japonica
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Summary:The carbohydrate specificity of three novel lectins, Boletopsis leucomelas lectin (BLL), Aralia cordate lectin (ACL), and Wasabia japonica lectin (WJL), was examined by frontal affinity chromatography using a panel of fluorescently labeled 47 oligosaccharides. The results indicate that BLL recognizes an agalacto structure of the biantennary chain and its bisecting structure. ACL showed strong affinity for triantennary oligosaccharides, but no affinity for tetraantennary structure. WJL showed no appreciable affinity for any of the 47 glycans examined. These lectins with a unique affinity specificity might be useful for examining alterations in the glycan structures of the glycoconjugates in association with development and various diseases.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.70.542