The Transport of Nanosized Gene Carriers Unraveled by Live-Cell Imaging

Tracking transport: Cationic polyethylenimine nanoparticles serve as nonviral vectors and deliver DNA to the nucleus of cells. Fluorescence live‐cell imaging highlights the interactions between these polyplexes and the cytoskeleton during the subsequent stages of transfection (see picture). The anal...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2006-02, Vol.45 (10), p.1568-1572
Main Authors: Bausinger, Ralf, von Gersdorff, Katharina, Braeckmans, Kevin, Ogris, Manfred, Wagner, Ernst, Bräuchle, Christoph, Zumbusch, Andreas
Format: Article
Language:English
Subjects:
Biological Transport
Cell Line, Tumor
Cell Survival
Diffusion
DNA
DNA - metabolism
fluorescence microscopy
gene expression
Gene Transfer Techniques
Humans
intracellular transport
nanoparticles
Nanostructures - analysis
Nanostructures - chemistry
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Summary:Tracking transport: Cationic polyethylenimine nanoparticles serve as nonviral vectors and deliver DNA to the nucleus of cells. Fluorescence live‐cell imaging highlights the interactions between these polyplexes and the cytoskeleton during the subsequent stages of transfection (see picture). The analysis of the underlying transport processes gives new impulse to further chemical‐synthesis approaches.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200503021