Inhibitory effects of hot water extract of the stevia [Stevia rebaudiana] stem on the contractile response of the smooth muscle of the guinea pig ileum

The effects of a hot water extract of the stem of Stevia rebaudiana on the smooth muscle of isolated guinea pig ileum were investigated. The butyl alcohol layer of the extract antagonized the contractions of the isolated guinea pig ileum induced by histamine (1 x 10E-5 M) and acetylcholine (1 x 10E-...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-02, Vol.70 (2), p.489-494
Main Authors: Shiozaki, K.(Tohoku Univ., Sendai (Japan)), Fujii, A, Nakano, T, Yamaguchi, T, Sato, M
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Animals
ANTAGONISM
ANTAGONISME
ANTAGONISMO
Biological and medical sciences
Butanols
calcium channel blocker: spasmolytic
Chromatography, High Pressure Liquid
COBAYA
COBAYE
Diterpenes, Kaurane - chemistry
Diterpenes, Kaurane - pharmacology
EXTRACTOS VEGETALES
EXTRAIT D'ORIGINE VEGETALE
Fundamental and applied biological sciences. Psychology
Glucosides - chemistry
Glucosides - pharmacology
GUINEA PIGS
Hot Temperature
Ileum - drug effects
Ileum - physiology
INTESTIN
INTESTINES
INTESTINOS
Male
Mass Spectrometry
MEDICINAL PROPERTIES
MERMA
Molecular Weight
Muscle Contraction - drug effects
Muscle Contraction - physiology
Muscle, Smooth - drug effects
Muscle, Smooth - physiology
PLANT EXTRACTS
Plant Extracts - pharmacology
Plant Shoots - chemistry
PROPIEDADES MEDICINALES
PROPRIETE PHARMACOLOGIQUE
RETRAIT
SHRINKAGE
Stevia - chemistry
STEVIA REBAUDIANA
stevioside
Water - chemistry
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Summary:The effects of a hot water extract of the stem of Stevia rebaudiana on the smooth muscle of isolated guinea pig ileum were investigated. The butyl alcohol layer of the extract antagonized the contractions of the isolated guinea pig ileum induced by histamine (1 x 10E-5 M) and acetylcholine (1 x 10E-5 M) in a concentration-dependent manner. The butyl alcohol layer of the extract also showed inhibition of CaClsub(2) (1 x 10E-3-3.8 x 10E-lM)-induced contractions. The antagonism of the extract was considered to be non-specific, but this action might be related to an influx of extracellular Casup(2+). With column chromatography preparation, the active component was assumed to be as stevioside. The antagonistic effects exerted by the stem extract of Stevia rebaudiana contributed to the gastroprotective activity of the extract in animals fed dietary histamine.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.70.489