Expression of the cannabinoid CB2 receptor in the rat cerebellum: An immunohistochemical study

Reports of cannabinoid CB2 receptor protein in the brain have been ambiguous. We therefore tested for CB2 immunoreactivity in the rat brain using immunofluorescence. We detected CB2 labeling in fine fibers in the granule layer. This CB2 labeling did not co-localise with the astrocyte marker glial fi...

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Bibliographic Details
Published in:Neuroscience letters 2006-03, Vol.396 (2), p.113-116
Main Authors: Ashton, John C., Friberg, Deborah, Darlington, Cynthia L., Smith, Paul F.
Format: Article
Language:English
Subjects:
Animals
Astrocytes - metabolism
Biological and medical sciences
Cannabinoid
Capillaries - metabolism
CB2 receptor
Cerebellum
Cerebellum - metabolism
Fundamental and applied biological sciences. Psychology
GFAP
Immunohistochemistry
Neurons - metabolism
Rats
Receptor, Cannabinoid, CB2 - metabolism
Tissue Distribution
Vertebrates: nervous system and sense organs
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Summary:Reports of cannabinoid CB2 receptor protein in the brain have been ambiguous. We therefore tested for CB2 immunoreactivity in the rat brain using immunofluorescence. We detected CB2 labeling in fine fibers in the granule layer. This CB2 labeling did not co-localise with the astrocyte marker glial fibrillary acidic protein (GFAP) and, therefore, the CB2-positive fibers were not astrocytes and were possibly microglial or neuronal. Additionally, strong CB2 labeling was detected in capillary endothelia in the granule, Purkinje cell, and molecular layers. Our results suggest that the role of CB2 receptors in the brain may have been previously underestimated.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2005.11.038