Induction of zinc transporters by forskolin in human trophoblast BeWo cells

During pregnancy, the zinc level in fetal serum is up to two-fold higher than that in the maternal serum at the end of pregnancy, but the mechanism of zinc release from the placenta into fetal circulation is not well understood. In this study, we determined the expression profiles of zinc transporte...

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Published in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2006-04, Vol.21 (3), p.285-291
Main Authors: Asano, Nagayoshi, Kondoh, Masuo, Ebihara, Chiaki, Fujii, Makiko, Nakanishi, Tsuyoshi, Utoguchi, Naoki, Enomoto, Shuichi, Tanaka, Keiichi, Watanabe, Yoshiteru
Format: Article
Language:English
Subjects:
BeWo cells
Biological and medical sciences
Cation Transport Proteins - biosynthesis
Cation Transport Proteins - genetics
Cation Transport Proteins - metabolism
Cell Differentiation
Cell Line
Chemical and industrial products toxicology. Toxic occupational diseases
Chorionic Gonadotropin - metabolism
Colforsin - pharmacology
Dose-Response Relationship, Drug
Embryology: invertebrates and vertebrates. Teratology
Female
Forskolin
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Humans
Medical sciences
Metals and various inorganic compounds
Placenta - metabolism
Pregnancy
RNA, Messenger - metabolism
Teratology. Teratogens
Time Factors
Toxicology
Trophoblasts - cytology
Trophoblasts - drug effects
Trophoblasts - metabolism
Up-Regulation
Zinc
Zinc transporter
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Summary:During pregnancy, the zinc level in fetal serum is up to two-fold higher than that in the maternal serum at the end of pregnancy, but the mechanism of zinc release from the placenta into fetal circulation is not well understood. In this study, we determined the expression profiles of zinc transporters in human trophoblast BeWo cells, a representative human trophoblast cell line. Zn transporter 1–8 (ZnT1–8), Zrt/IRT-like protein 1 and Zn transporter-like transporter 1 were detected in BeWo cells by reverse transcription-polymerase chain reaction. Forskolin (FK) is a representative inducer of differentiation of BeWo cells cytotrophoblast into syncytiotrophoblast. Treatment of BeWo cells with FK resulted in morphological changes of BeWo cells into syncytiotrophoblast cells and secretion of human chorionic gonadotropin, which is a characteristic of syncytiotrophoblast cells. Treatment of BeWo cells with FK elevated ZnT1, 2 and 4 mRNA levels. These data about expression profiles of ZnTs may be useful for further investigation of placental biology.
ISSN:0890-6238
1873-1708
DOI:10.1016/j.reprotox.2005.02.006