Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery

Frontal affinity chromatography coupled with MS (FAC-MS) and its application in a broad range of biological systems, ability to rank ligands and determine Kd makes it a universal tool in drug discovery. The emergence of a relatively new technique resulting from a combination of frontal affinity chro...

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Bibliographic Details
Published in:Drug Discovery Today 2005-03, Vol.10 (6), p.409-416
Main Authors: Slon-Usakiewicz, Jacek J., Ng, William, Dai, Jin-Rui, Pasternak, Andrew, Redden, Peter R.
Format: Article
Language:English
Subjects:
binding assay
Binding Sites
Biological and medical sciences
Chromatography, Affinity
Drug Design
drug discovery
Drug Evaluation, Preclinical - methods
FAC-MS
General pharmacology
HTS
Ligands
Mass Spectrometry
Medical sciences
Models, Molecular
Molecular Conformation
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
protein immobilization
Proteins - chemistry
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Summary:Frontal affinity chromatography coupled with MS (FAC-MS) and its application in a broad range of biological systems, ability to rank ligands and determine Kd makes it a universal tool in drug discovery. The emergence of a relatively new technique resulting from a combination of frontal affinity chromatography coupled with MS detection (FAC-MS) has extended the capabilities of MS in drug discovery and development. Its application in a broad range of biological systems, together with its label-free operation, relatively high throughput, ability to rank ligands and determine K d, makes FAC-MS a universal tool enabling convenient and efficient screening in the identification of new potential drug leads. Here we will highlight FAC-MS screening studies and discuss where it can be applied in evaluating multiple protein-binding sites, protein-protein interactions and inactive proteins, and also in determining selectivity.
ISSN:1359-6446
1878-5832
DOI:10.1016/S1359-6446(04)03360-4