Parkin enhances mitochondrial biogenesis in proliferating cells

We describe a novel function of parkin, a RING protein, which is elaborately involved in mitochondrial biogenesis. Parkin was located within the mitochondrial organelle of proliferating cells. Anti-proliferative treatments released parkin from mitochondria to cytosol. Results of pharmacological trea...

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Bibliographic Details
Published in:Human molecular genetics 2006-03, Vol.15 (6), p.883-895
Main Authors: Kuroda, Yukiko, Mitsui, Takao, Kunishige, Makoto, Shono, Masayuki, Akaike, Masashi, Azuma, Hiroyuki, Matsumoto, Toshio
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line, Tumor
Cell Proliferation
Cercopithecus aethiops
COS Cells
DNA-Binding Proteins - metabolism
Fundamental and applied biological sciences. Psychology
Gene Silencing
Genetics of eukaryotes. Biological and molecular evolution
Humans
Microscopy, Immunoelectron
Mitochondria - genetics
Mitochondria - metabolism
Mitochondria - secretion
Mitochondria - ultrastructure
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Mitochondrial Proteins - physiology
Mitochondrial Proteins - secretion
Molecular and cellular biology
Protein Transport - genetics
Transcription Factors - metabolism
Transcription, Genetic - physiology
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Ubiquitin-Protein Ligases - physiology
Ubiquitin-Protein Ligases - secretion
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Summary:We describe a novel function of parkin, a RING protein, which is elaborately involved in mitochondrial biogenesis. Parkin was located within the mitochondrial organelle of proliferating cells. Anti-proliferative treatments released parkin from mitochondria to cytosol. Results of pharmacological treatments indicate that parkin was released from mitochondria when permeability transition pore was opened. The extra-mitochondrial localization was also observed in differentiated cells. In proliferating cells, transcription and replication of mitochondrial DNA was enhanced by parkin overexpression and attenuated by parkin suppression with siRNA. Parkin was associated with mitochondrial transcription factor A (TFAM) and enhanced TFAM-mediated mitochondrial transcription. These results indicate that parkin is involved in the regulation of mitochondrial transcription/replication other than the ubiquitin-mediated protein degradation system in proliferating cells.
ISSN:0964-6906
1460-2083
DOI:10.1093/hmg/ddl006