Transplantation of hepatocytes: Temporary elimination of scavenger cells prevents early loss of grafted cells

Transplants of isolated syngeneic and allogeneic hepatocytes are rapidly disintegrated, irrespective of the site of engraftment; be it spleen, liver, portal vein, peritoneum, or subcutaneous tissue. Host scavenger cells are responsible for this reaction. We designed a method overcoming early disinte...

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Bibliographic Details
Published in:Transplantation proceedings 2005, Vol.37 (1), p.260-261
Main Authors: Wesolowska, A., Olszewski, W.L., Durlik, M.
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Transplantation
Hepatocytes - transplantation
Liver Transplantation
Rats
Rats, Inbred Lew
Stem Cell Transplantation
Tissue Transplantation - methods
Transplantation Conditioning
Whole-Body Irradiation
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Summary:Transplants of isolated syngeneic and allogeneic hepatocytes are rapidly disintegrated, irrespective of the site of engraftment; be it spleen, liver, portal vein, peritoneum, or subcutaneous tissue. Host scavenger cells are responsible for this reaction. We designed a method overcoming early disintegration of the grafted hepatocytes. It consisted of administration of anti-asialoGM1 antiserum eliminating natural killer cells; sublethal whole body irradiation; and reconstitution with syngeneic bone marrow cells, ligation of host bile duct, intrasplenic hepatocyte transplantation, and three consecutive partial hepatectomies. Six months after transplantation a glycogen-rich, trabeculae-forming, dividing hepatocytes, situated along strands of newly-formed fibrous tissue and numerous dilated blind bile cannaliculae were observed. There was evidently more bile canaliculae in hosts with ligated bile duct than nonligated controls. This is the first study showing fibrous tissue formed at the site of hepatocyte implantation, and stellate cells are presumably involved in this process.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2004.12.160