Techniques: high-throughput measurement of intracellular Ca(2+) -- back to basics

High-throughput screening techniques continue to provide important tools to the pharmaceutical industry for the efficient identification of drug leads. However, high-throughput techniques are now being exploited to address a variety of pharmacological and cellular signaling research questions, inclu...

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Bibliographic Details
Published in:Trends in pharmacological sciences (Regular ed.) 2005-04, Vol.26 (4), p.218-223
Main Authors: Monteith, Gregory R, Bird, Gary St J
Format: Article
Language:English
Subjects:
Animals
Calcium - analysis
Calcium Signaling
Cells, Cultured
Computational Biology - methods
Drug Design
Fluorometry
GTP-Binding Proteins - metabolism
Humans
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Summary:High-throughput screening techniques continue to provide important tools to the pharmaceutical industry for the efficient identification of drug leads. However, high-throughput techniques are now being exploited to address a variety of pharmacological and cellular signaling research questions, including the regulation and role of intracellular Ca(2+) in a plethora of biological systems. Although an awareness of specific assay conditions is crucial for reliable and reproducible measurements of intracellular free Ca(2+) whichever system of study is used, the complex temporal nature of Ca(2+) signals has posed some unique limitations for its measurement in high-throughput mode. Progress in high-throughput design has overcome many of these limitations and will complement other technical approaches to understanding the underlying regulation and role of intracellular Ca(2+).
ISSN:0165-6147
DOI:10.1016/j.tips.2005.02.002