Prevalence of antiphospholipid antibodies, factor V G1691A (Leiden) and prothrombin G20210A mutations in early and late recurrent pregnancy loss

• Published in: European journal of obstetrics & gynecology and reproductive biology 2005-04, Vol.119 (2), p.164-170
• Main Authors: Mtiraoui, Nabil, Borgi, Lobna, Hizem, Sondes, Nsiri, Brahim, Finan, Ramzi R., Gris, Jean-Christophe, Almawi, Wassim Y., Mahjoub, Touhami
• Format: Article
• Language: English
• Subjects: Abortion, Habitual - genetics; Abortion, Habitual - immunology; Activated Protein C Resistance - epidemiology; Adolescent; Adult; Antibodies, Antiphospholipid - blood; Antiphospholipid antibodies; Biological and medical sciences; Diseases of mother, fetus and pregnancy; Factor V - analysis; Factor V - genetics; Factor V-Leiden; Female; Genotype; Gestational Age; Gynecology. Andrology. Obstetrics; Hematologic and hematopoietic diseases; Humans; Medical sciences; Mutation; Platelet diseases and coagulopathies; Pregnancy; Pregnancy. Fetus. Placenta; Prothrombin - genetics; Prothrombin G20210A mutation; Recurrent abortion; Tunisia
• ISSN: 0301-2115; 1872-7654
• DOI: 10.1016/j.ejogrb.2004.07.003

: We assessed the prevalence of inherited (FV-Leiden and PRT G20210A), and acquired (anti-PL antibodies) risk factors among habitual aborters in Tunisia. : We studied prospectively 146 patients with ≥3 consecutive early, late, or early–late recurrent pregnancy losses, together with 99 age-matched controls. Anticardiolipin antibodies (ACL), lupus anticoagulant (LA), and APC resistance (APCR) were detected by ELISA, dilute Russell Viper Venom Time (dRVVT), and coagulation tests, respectively, and FV-Leiden and PRT G20210A genotypes were assessed by PCR. : Anti-PL antibody frequencies were 45 and 9% among patients and controls, respectively ( P < 0.001), with positive LA only ( P = 0.004), or combined elevated ACL-positive LA being consistently higher ( P < 0.001) among patients than controls. FV-Leiden (20.54% versus 6.06%), but not PRT G20210A (2.74% versus 4.04%) was significantly higher in patients versus controls. Among LA-positive cases higher prevalence of G/A (14/146 versus 1/99) and A/A genotypes (4/146 versus 0/99) were seen, and among ACL-positive cases higher prevalence of G/A (10/146 versus 0/99) and A/A genotypes (2/146 versus 0/99) were recorded. : Anti-PL antibodies and FV-Leiden, but not PRT G20210A, are associated with recurrent idiopathic pregnancy losses in Tunisian women.