Evaluation of the stool antigen Test for Helicobacter pylori in children and adolescents

The stool antigen test for Helicobacter pylori is a noninvasive immunoassay to diagnose active infection with Helicobacter pylori in human fecal samples. Its performance in children and teenagers has been tested in some developed countries, showing a sensitivity and specificity above 90%, however, i...

Full description

Saved in:
Bibliographic Details
Published in:Digestive diseases and sciences 2005-03, Vol.50 (3), p.453-457
Main Authors: RAGUZA, Daniele, GRANATO, Celso Francisco H, KAWAKAMI, Elisabete
Format: Article
Language:English
Subjects:
Adolescent
Age Distribution
Antigens, Bacterial - analysis
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Biological and medical sciences
Chi-Square Distribution
Child
Child, Preschool
Cohort Studies
Confidence Intervals
Feces - microbiology
Female
Helicobacter Infections - diagnosis
Helicobacter Infections - epidemiology
Helicobacter Infections - immunology
Helicobacter pylori
Helicobacter pylori - immunology
Helicobacter pylori - isolation & purification
Human bacterial diseases
Humans
Immunologic Techniques
Incidence
Infant
Infectious diseases
Male
Medical sciences
Odds Ratio
Risk Assessment
Sensitivity and Specificity
Sex Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The stool antigen test for Helicobacter pylori is a noninvasive immunoassay to diagnose active infection with Helicobacter pylori in human fecal samples. Its performance in children and teenagers has been tested in some developed countries, showing a sensitivity and specificity above 90%, however, its accuracy in developing countries and in children under 6 years is not well established. To determine the accuracy of the test for diagnosing Helicobacter pylori infection in children and teenagers, we evaluated 133 patients (4 months to 17 years old). The gold standard was a positive culture or positive histology and rapid urease test. The test was done according to the manufacturer's instructions. However, modifications were introduced for better reproducibility. Samples were analyzed twice and results are expressed as optical density (OD) determined spectrophotometrically at 450 nm. HpSA was considered positive at OD > or =0.160 and negative at OD
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-005-2457-4