Periodontitis Deteriorates Metabolic Control in Type 2 Diabetic Goto‐Kakizaki Rats

• Published in: Journal of periodontology (1970) 2006-03, Vol.77 (3), p.350-356
• Main Authors: Pontes Andersen, Carla C., Buschard, Karsten, Flyvbjerg, Allan, Stoltze, Kaj, Holmstrup, Palle
• Format: Article
• Language: English
• Subjects: Adipose Tissue - metabolism; Alveolar Bone Loss - etiology; Analysis of Variance; Animal models; Animals; Blood Glucose - analysis; Cytokines - analysis; Dentistry; diabetes; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - metabolism; Disease Models, Animal; Fatty Acids, Nonesterified - blood; Glucose Intolerance - complications; Insulin - blood; Insulin Resistance; Ligation; Male; periodontitis; Periodontitis - complications; Rats; Rats, Inbred Strains; Rats, Wistar; Statistics, Nonparametric
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2006.050184

Background: Epidemiologic and clinical studies have indicated that periodontal disease (PD) may cause disturbances in general health and even affect diabetes. The aim of this study was to gain knowledge on the effect of PD on diabetes metabolic control in a new model for type 2 diabetes–associated PD (i.e., the Goto‐Kakizaki [GK] rat). Methods: GK rats represented the type 2 diabetes group and were allocated into two groups: diabetes or diabetes + PD group; Wistar rats represented the non‐diabetes group and were divided into non‐diabetes + PD and non‐diabetes groups. PD was induced by placing ligatures around second maxillary molars, and the animals were followed for 6 weeks. Serum insulin, glucose, and free fatty acid levels were evaluated; interleukin (IL)‐1β, IL‐6, and tumor necrosis factor‐α were measured in adipose tissue supernatant; glucose tolerance and insulin resistance were calculated. Further, alveolar bone destruction was estimated morphometrically and radiographically. Results: Rats with diabetes + PD became almost 30% more glucose intolerant (P