Expression and immunohistochemical localization of Cdc2 and P70S6K in different stages of mouse germ cells

In order to determine the function and possible relationship between Cdc2 and P70S6K, Western blot analysis and immunohistochemistry analysis were used to study the expression and kinase activity of Cdc2 and P70S6K in male mouse germ cells. With the maturation of germ cells in the testis, the expres...

Full description

Saved in:
Bibliographic Details
Published in:Cell biochemistry and function 2006-03, Vol.24 (2), p.113-117
Main Authors: Yu, Bing-Zhi, Song, Yu-Tong, Yu, Da-Hai, Su, Wen-Hui, Gasana, Venuste, Li, Yan-Xiao, Zhang, Zhe
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Cdc2
CDC2 Protein Kinase - analysis
CDC2 Protein Kinase - biosynthesis
cell growth
cell reproduction
Gene Expression Regulation, Developmental
Immunohistochemistry
Male
Maturation-Promoting Factor - physiology
Mice
P70S6K
Ribosomal Protein S6 Kinases, 70-kDa - analysis
Ribosomal Protein S6 Kinases, 70-kDa - biosynthesis
Spermatocytes - enzymology
Spermatozoa - enzymology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to determine the function and possible relationship between Cdc2 and P70S6K, Western blot analysis and immunohistochemistry analysis were used to study the expression and kinase activity of Cdc2 and P70S6K in male mouse germ cells. With the maturation of germ cells in the testis, the expression of Cdc2 and P70S6K was relatively constant. However, the kinase activity of P70S6K was increased and the phosphorylation of Tyr15 residue of Cdc2 was enhanced, which suggests that the kinase activity of Cdc2 is decreasing. Immunohistochemistry analysis also showed that there was a P70S6K transfer from nucleus to cytoplasm during spermatogenesis. During spermatogenesis, cell division of the germ cell in male mouse is decelerated; nevertheless, cell growth is enhanced. Cdc2 and P70S6K are involved in these two processes. It could be an alternative mechanism to prepare for future fertilization that Cdc2 is able to maintain a subtle balance between the production and growth of male germ cells by regulating P70S6K. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.1306