Degradation of cyclins D in pseudorabies virus (PRV) infected proliferating cells

• Published in: Veterinary microbiology 2006-03, Vol.113 (3), p.179-184
• Main Authors: Blanchard, Yannick, Dory, Daniel, Briand, François Xavier, Félix, Hélène, Jestin, André
• Format: Article
• Language: English
• Subjects: Animals; Aujeszky disease; Biological and medical sciences; Blotting, Western - veterinary; cell proliferation; Cells, Cultured; Cyclin B; Cyclin B - metabolism; Cyclin D; Cyclin D1 - metabolism; cyclins; Cyclins - metabolism; Flow Cytometry - veterinary; Fluorescence; Fundamental and applied biological sciences. Psychology; HeLa Cells; Herpes simplex virus 1; Herpesvirus 1, Human - genetics; Herpesvirus 1, Human - metabolism; Herpesvirus 1, Human - pathogenicity; Herpesvirus 1, Suid - genetics; Herpesvirus 1, Suid - metabolism; Herpesvirus 1, Suid - pathogenicity; Humans; ICP0; Immediate-Early Proteins - metabolism; infection; Microbiology; Miscellaneous; Molecular Weight; protein degradation; protein stability; protein structure; Pseudorabies virus; Suid herpesvirus 1; Ubiquitin-Protein Ligases - metabolism; viral proteins; Virology; Virulence - genetics
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2005.11.011

The pseudorabies virus code for an ICP0 protein which is half the size of the HSV1 ICP0 protein. In this work, we made the assumption that some function might have been lost in the ICP0 from PRV. One function attributed to the ICP0 from HSV1 was the stabilization of cyclins D. We then looked at the stability of these cyclins during the lytic infection with the PRV. Our results show that cyclins D are not stabilized during infection with the PRV. These results are in accord with recent data from the literature.