Nodavirus infection causes mortalities in hatchery produced larvae of Lates calcarifer: first report from India

Larvae (15 to 21 d post hatch, dph) of the Asian sea bass Lates calcarifer (Bloch) suffered heavy mortalities (60 to 90%) during the hatchery-rearing phase. Darkened and moribund larvae showed no evidence of bacterial or parasitic infections. Tissue sections of brain and spinal cord showed clear nec...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2005-02, Vol.63 (2-3), p.113-118
Main Authors: AZAD, I. S, SHEKHAR, M. S, THIRUNAVUKKARASU, A. R, POORNIMA, M, KAILASAM, M, RAJAN, J. J. S, ALI, S. A, ABRAHAM, M, RAVICHANDRAN, P
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Animals
Aquaculture
Biological and medical sciences
Capsid Proteins - genetics
Cytoplasm - virology
DNA Primers
Fish Diseases - epidemiology
Fish Diseases - mortality
Fish Diseases - virology
Fundamental and applied biological sciences. Psychology
India - epidemiology
Larva - ultrastructure
Larva - virology
Marine
Microscopy, Electron - veterinary
Neurons - ultrastructure
Neurons - virology
Nodaviridae
Perciformes
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA Virus Infections - epidemiology
RNA Virus Infections - mortality
RNA Virus Infections - veterinary
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
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Summary:Larvae (15 to 21 d post hatch, dph) of the Asian sea bass Lates calcarifer (Bloch) suffered heavy mortalities (60 to 90%) during the hatchery-rearing phase. Darkened and moribund larvae showed no evidence of bacterial or parasitic infections. Tissue sections of brain and spinal cord showed clear necrotic vacuolation. Electron microscopy revealed membrane-bound viral particles in the cytoplasm of the nerve cells. The viral particles measured 28 to 30 nm in diameter. Primer sets, designed for the amplification of the RNA2 segment of the piscine nodavirus coat protein gene, were used in the RT-PCR analysis of moribund larvae of 20 and 21 dph which produced the amplified product of 430 bp. The clinical manifestations, pathology and electron microscopy observations supported by the RT-PCR analysis suggest that the nerve necrosis was due to nodavirus infection in the larvae. This is the first report of piscine nodavirus infection from the Indian sub-continent.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao063113