Screening the whole genome of a pathogen in vivo for individual protective antigens

We report the results of a general protocol that was used to screen the whole genome of Chlamydophila abortus, type strain B577 ( formerly Chlamydia psittaci strain B577), in a mouse pneumonia model. Genetic immunization was used to functionally test the genes of C. abortus as vaccines in a mouse ch...

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Bibliographic Details
Published in:Vaccine 2005-04, Vol.23 (23), p.3016-3025
Main Authors: Stemke-Hale, Katherine, Kaltenboeck, Bernhard, DeGraves, Fred J., Sykes, Kathryn F., Huang, Jin, Bu, Chun-hui, Johnston, Stephen Albert
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Applied microbiology
Bacterial Vaccines - immunology
Bacteriology
Bioinformatics
Biological and medical sciences
Candidates
Cardiovascular disease
Chlamydia
Chlamydia psittaci
Chlamydophila - genetics
Chlamydophila - immunology
Chlamydophila abortus
Cloning
Deoxyribonucleic acid
DNA
Expression library immunization
Female
Fertility
Fundamental and applied biological sciences. Psychology
Gene Library
Genes
Genome, Bacterial
Genomes
Histocompatibility Antigens Class II - immunology
Immunization
Infertility
Mice
Microbiology
Miscellaneous
Pathogens
Peptides
Plasmids
Pneumonia
Proteins
Sexually transmitted diseases
STD
Vaccination
Vaccine
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, DNA - immunology
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Summary:We report the results of a general protocol that was used to screen the whole genome of Chlamydophila abortus, type strain B577 ( formerly Chlamydia psittaci strain B577), in a mouse pneumonia model. Genetic immunization was used to functionally test the genes of C. abortus as vaccines in a mouse challenge system. Nine gene fragments were isolated that conferred protection, with five protecting as effectively as the live-vaccine positive control. Bioinformatics approaches were unable to reconstruct isolation of these antigens. These results suggest that pathogen genomes can be functionally screened for vaccine candidate antigens in a mouse model to reveal new classes of vaccine candidate antigens that may have therapeutic efficacy across host species, disease manifestations, and delivery platforms.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2004.12.013