Preparation of Liposomes Using an Improved Supercritical Reverse Phase Evaporation Method

Liposomes of various phospholipids were prepared using an improved supercritical reverse phase evaporation (ISCRPE) method that utilizes supercritical carbon dioxide (scCO2) as an alternative to organic solvents. Using this method, in the absence of any organic solvent including ethanol, the maximum...

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Bibliographic Details
Published in:Langmuir 2006-03, Vol.22 (6), p.2543-2550
Main Authors: Otake, Katsuto, Shimomura, Takeshi, Goto, Toshihiro, Imura, Tomohiro, Furuya, Takeshi, Yoda, Satoshi, Takebayashi, Yoshihiro, Sakai, Hideki, Abe, Masahiko
Format: Article
Language:English
Subjects:
Chemistry
Colloidal state and disperse state
Exact sciences and technology
General and physical chemistry
Membranes
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Summary:Liposomes of various phospholipids were prepared using an improved supercritical reverse phase evaporation (ISCRPE) method that utilizes supercritical carbon dioxide (scCO2) as an alternative to organic solvents. Using this method, in the absence of any organic solvent including ethanol, the maximum trapping efficiency of glucose reached 36% for 20 mM l-α-dioleoylphosphatidylcholine (DOPC), compared to less than 10% using the Bangham method. Liposomes prepared by the ISCRPE method were highly stable for one month at room temperature. Freeze fractured TEM observations, osmotic shrinkage measurements, and DSC measurements revealed that the liposomes prepared by the ISCRPE method are unilamellar vesicles with loosely packed phospholipids. Comparison of nitrogen with scCO2 revealed that the presence of CO2 is necessary for the formation of liposomes.
ISSN:0743-7463
1520-5827
DOI:10.1021/la051654u