Proteome analysis of glandular parotid and submandibular-sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis

The secretions of the salivary parotid and submandibular‐sublingual (SMSL) glands constitute the main part of whole human saliva (WS) in which proline‐rich proteins (PRPs) and mucins represent dominant groups. Although proteome analysis had been performed on WS, no identification of PRPs or mucins b...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2006-03, Vol.6 (5), p.1631-1639
Main Authors: Walz, Anke, Stühler, Kai, Wattenberg, Andreas, Hawranke, Eva, Meyer, Helmut E., Schmalz, Gottfried, Blüggel, Martin, Ruhl, Stefan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Humans
Miscellaneous
Molecular Sequence Data
Mucins - analysis
Parotid Gland - secretion
Peptides - analysis
Proline-Rich Protein Domains
Proline-rich proteins
Proteins
Proteome - analysis
Saliva
Saliva - chemistry
Saliva - secretion
Salivary glands
Salivary mucins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Submandibular Gland - secretion
Two-dimensional gel electrophoresis
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Summary:The secretions of the salivary parotid and submandibular‐sublingual (SMSL) glands constitute the main part of whole human saliva (WS) in which proline‐rich proteins (PRPs) and mucins represent dominant groups. Although proteome analysis had been performed on WS, no identification of PRPs or mucins by 2‐DE and MS was achieved in WS and no comprehensive analysis of both glandular secretions is available so far. The aim of this study was to compare the protein map of WS to parotid and SMSL secretions for the display of PRPs and mucins. WS and glandular secretions were subjected to 2‐DE and spots were analyzed by MALDI‐MS. New components identified in WS were cyclophilin‐B and prolyl‐4‐hydroxylase. Also acidic and basic PRPs as well as the proline‐rich glycoprotein (PRG) could now be mapped in WS. Acidic PRPs were found equally in parotid and SMSL secretions, whereas basic PRPs and PRG were found primarily in parotid secretion. Salivary mucin MUC7 was identified in SMSL secretion. Thus, the more abundant proteins of WS can be explained mainly by mixed contributions of parotid and SMSL secretions with only few components remaining that may be derived from local sources in the oral cavity.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200500125