Generation of Phenylpropanoid Pathway-Derived Volatiles in Transgenic Plants: Rose Alcohol Acetyltransferase Produces Phenylethyl Acetate and Benzyl Acetate in Petunia Flowers

• Published in: Plant molecular biology 2006-03, Vol.60 (4), p.555-563
• Main Authors: Guterman, I, Masci, T, Chen, X, Negre, F, Pichersky, E, Dudareva, N, Weiss, D, Vainstein, A
• Format: Article
• Language: English
• Subjects: Acetates - analysis; Acetates - chemistry; Acetates - metabolism; acyltransferases; alcohol acetyltransferase; Alcohols; benzyl acetate; Benzyl Alcohol - analysis; Benzyl Alcohol - chemistry; Benzyl Compounds - metabolism; biosynthesis; Chromatography, Gas; emissions; enzyme activity; Esters; Flowers; Flowers & plants; Flowers - genetics; Flowers - metabolism; gene expression; gene transfer; genes; Kinetics; Mass Spectrometry; odors; Petunia; Petunia - genetics; Petunia - metabolism; Petunia hybrida; phenylethyl acetate; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - analysis; Phenylethyl Alcohol - chemistry; Phenylethyl Alcohol - metabolism; Plant Leaves - metabolism; Plants, Genetically Modified; Propanols - metabolism; Proteins - genetics; Proteins - metabolism; RhAAT gene; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rosa; Rosa - enzymology; Rosa - genetics; Substrate Specificity; Terpenes - analysis; Terpenes - chemistry; Transgenic plants; Volatilization
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/s11103-005-4924-x

Esters are important contributors to the aroma of numerous flowers and fruits. Acetate esters such as geranyl acetate, phenylethyl acetate and benzyl acetate are generated as a result of the action of alcohol acetyltransferases (AATs). Numerous homologous AATs from various plants have been characterized using in-vitro assays. To study the function of rose alcohol acetyltransferase (RhAAT) in planta, we generated transgenic petunia plants expressing the rose gene under the control of a CaMV-35S promoter. Although the preferred substrate of RhAAT in vitro is geraniol, in transgenic petunia flowers, it used phenylethyl alcohol and benzyl alcohol to produce the corresponding acetate esters, not generated by control flowers. The level of benzyl alcohol emitted by the flowers of different transgenic lines was ca. three times higher than that of phenylethyl alcohol, which corresponded to the ratio between the respective products, i.e. ca. three times more benzyl acetate than phenylethyl acetate. Feeding of transgenic petunia tissues with geraniol or octanol led to the production of their respective acetates, suggesting the dependence of volatile production on substrate availability.