Intracytoplasmic glutathione levels in heifer oocytes cultured in different maturation media and its effect on embryo development

Contents The present study was carried out to study the effect of different maturation media on embryo development of heifer oocytes and on their glutathione (GSH) synthesis during in vitro maturation (IVM). Immature heifer oocytes were matured in parallel in one of four maturation media: (i) Tissue...

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Bibliographic Details
Published in:Reproduction in domestic animals 2005-04, Vol.40 (2), p.126-130
Main Authors: Mertens, M.J, Lopez-Bejar, M, Paramio, M.T
Format: Article
Language:English
Subjects:
age
age differences
Age Factors
Animal reproduction
Animals
Biological and medical sciences
Blastocyst
Cattle
Cattle - embryology
Cattle - physiology
cell culture
Cells
cows
culture media
Culture Media - chemistry
cytoplasm
embryo (animal)
embryo culture
embryogenesis
Embryonic Development - drug effects
Embryos
epidermal growth factor
Epidermal Growth Factor - pharmacology
Female
Fertilization in Vitro - veterinary
fetal bovine serum
Follicle Stimulating Hormone - pharmacology
follicle-stimulating hormone
food animals
Fundamental and applied biological sciences. Psychology
glutathione
Glutathione - biosynthesis
heifers
Mammalian reproduction. General aspects
oocytes
Oocytes - cytology
Oocytes - metabolism
Oocytes - physiology
Peptides
Tissue Culture Techniques
Vertebrates: reproduction
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Summary:Contents The present study was carried out to study the effect of different maturation media on embryo development of heifer oocytes and on their glutathione (GSH) synthesis during in vitro maturation (IVM). Immature heifer oocytes were matured in parallel in one of four maturation media: (i) Tissue Culture Medium (TCM)‐199 supplemented with 10 ng/ml of epidermal growth factor (EGF); (i) TCM‐199 supplemented with 10 ng/ml of EGF plus 1 μg/ml of FSH; (iii) TCM‐199 supplemented with 10% of foetal bovine serum (FBS) and (iv) TCM‐199 supplemented with 10% of FBS plus 1 μg/ml of FSH. Cow oocytes were used as control and were matured in TCM‐199 supplemented with 10 ng/ml of EGF. No differences were observed in blastocyst rate among the different heifer oocyte groups (8.8, 7.5. 8.4 and 6.8%, respectively) however, the percentage of blastocysts obtained from cow oocytes was significantly higher (30%; p 
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2005.00566.x