Cytosolic dehydroascorbate reductase is important for ozone tolerance in Arabidopsis thaliana

Dehydroascorbate reductase (DHAR) is a key component of the ascorbate recycling system. Three functional DHAR genes are encoded in the Arabidopsis genome. Ozone exposure increased the expression of the cytosolic DHAR (cytDHAR) gene alone. We characterized an Arabidopsis mutant with a deficient cytDH...

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Bibliographic Details
Published in:Plant and cell physiology 2006-02, Vol.47 (2), p.304-308
Main Authors: Yoshida, S.(Tsukuba Univ., Ibaraki (Japan)), Tamaoki, M, Shikano, T, Nakajima, N, Ogawa, D, Ioki, M, Aono, M, Kubo, A, Kamada, H, Inoue, Y, Saji, H
Format: Article
Language:English
Subjects:
ACIDE ASCORBIQUE
ACIDO ASCORBICO
Arabidopsis - enzymology
Arabidopsis - physiology
ARABIDOPSIS THALIANA
Arabidopsis thaliana— Apoplast —Ascorbate
AsA
ascorbate
ASCORBIC ACID
Ascorbic Acid - metabolism
Cytosol - enzymology
dehydroascorbate
Dehydroascorbate reductase
DHA
DHAR
ESTRES
ethylene
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genes, Plant
Glutathione - metabolism
Mutation
OXIDACION
OXIDATION
Oxidative stress
Oxidoreductases - metabolism
OXYDATION
OZONE
OZONO
photosynthetic photon flux density
PPFD
reactive oxygen species
reverse transcription–PCR
RNA, Messenger - analysis
RNA, Messenger - genetics
ROS
RT–PCR
STRESS
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Summary:Dehydroascorbate reductase (DHAR) is a key component of the ascorbate recycling system. Three functional DHAR genes are encoded in the Arabidopsis genome. Ozone exposure increased the expression of the cytosolic DHAR (cytDHAR) gene alone. We characterized an Arabidopsis mutant with a deficient cytDHAR. The mutant completely lacked cytDHAR activity and was highly ozone sensitive. The amounts of total ascorbate and glutathione were similar in both lines, but the amount of apoplastic ascorbate in the mutant was 61.5% lower. These results indicate that the apoplastic ascorbate, which is generated through the reduction of DHA by cytDHAR, is important for ozone tolerance.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pci246