Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022

The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int cat...

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Bibliographic Details
Published in:Plant molecular biology 2005-02, Vol.57 (3), p.435-444
Main Authors: Gottfried, P, Lotan, O, Kolot, M, Maslenin, L, Bendov, R, Gorovits, R, Yesodi, V, Yagil, E, Rosner, A
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Arabidopsis - genetics
Arabidopsis thaliana
Bacteriophage HK022 - enzymology
bacteriophages
Binding Sites - genetics
Blotting, Northern
Blotting, Southern
Catalysis
Cell Nucleus - metabolism
coliphage HK022
DNA, Plant - genetics
DNA, Plant - metabolism
Enzymes
Gene expression
Gene Expression Regulation, Enzymologic
gene transfer
genetic recombination
genetic transformation
green fluorescent protein
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Immunoblotting
int gene
Integrase protein
Integrases - genetics
Integrases - metabolism
Microscopy, Fluorescence
Nicotiana - genetics
Nicotiana benthamiana
Phage HK022
Plant biology
Plants, Genetically Modified
Proteins
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Recombination, Genetic - genetics
reporter genes
site-specific recombination
Tobacco
transgenes
Transgenic plants
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP x attB) as well as a site-specific excision reaction (attL x attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-004-0076-7