The oxidative stress response of the yeast Candida intermedia to copper, zinc, and selenium exposure

The yeast Candida intermedia, as a model organism, was used to examine the links between the metal ions exposure, reactive oxygen species generation and oxidative stress response. To estimate intracellular peroxide and superoxide levels, the fluorescence indicators dihydrorhodamine 123 and dihydroet...

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Bibliographic Details
Published in:Journal of basic microbiology 2005-04, Vol.45 (2), p.125-135
Main Authors: Fujs, Štefan, Gazdag, Zoltán, Poljšak, Borut, Stibilj, Vekoslava, Milačič, Radmila, Pesti, Miklós, Raspor, Peter, Batič, Martin
Format: Article
Language:English
Subjects:
Candida - drug effects
Candida - growth & development
Candida - metabolism
Candida intermedia
Copper - pharmacology
Culture Media
Glutathione - analysis
Glutathione - metabolism
Glutathione Peroxidase - metabolism
Microbial Sensitivity Tests
Oxidative Stress
Reactive Oxygen Species - metabolism
Selenium - pharmacology
Superoxide Dismutase - metabolism
Time Factors
Zinc - pharmacology
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Summary:The yeast Candida intermedia, as a model organism, was used to examine the links between the metal ions exposure, reactive oxygen species generation and oxidative stress response. To estimate intracellular peroxide and superoxide levels, the fluorescence indicators dihydrorhodamine 123 and dihydroethidium were used, respectively. Antioxidant defence systems were investigated by measuring the activity of catalase, glutathione peroxidase and superoxide dismutase and the content of reduced glutathione. Altered superoxide, peroxide, glutathione levels, and the catalase activity were perceived after the treatment with copper. In the samples treated with selenium and zinc the altered peroxide and superoxide levels, and the glutathione peroxidase activity were perceived. The results indicate that the tolerance of the yeast C. intermedia to different metal ions was correlated with the reactive oxygen species generation in the cells and with the efficiency of antioxidative defence systems. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.200410480