Oceanimonas smirnovii sp. nov., a novel organism isolated from the Black Sea

A slightly creamy, melanogenic, Gram-negative, aerobic bacterium was isolated from seawater sample collected in the Karadag Natural Reserve of the Eastern Crimea, the Black Sea. The novel organism was chemoorganotrophic, had no obligate requirement in NaCl, tolerated to 12% NaCl, grew between 10 and...

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Bibliographic Details
Published in:Systematic and applied microbiology 2005-03, Vol.28 (2), p.131-136
Main Authors: Ivanova, Elena P., Onyshchenko, Olga M., Christen, Richard, Zhukova, Natalia V., Lysenko, Anatoly M., Shevchenko, Ludmila S., Buljan, Vlado, Hambly, Brett, Kiprianova, Elena A.
Format: Article
Language:English
Subjects:
Aeromonadaceae - chemistry
Aeromonadaceae - classification
Aeromonadaceae - genetics
Aeromonadaceae - isolation & purification
Bacterial Typing Techniques
Base Composition
Brackish
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
Fatty Acids - analysis
Genes, rRNA
Lipids - analysis
Molecular Sequence Data
Nucleic Acid Hybridization
Oceanimonas smirnovii
Phenotype
Phylogeny
RNA, Ribosomal, 16S - genetics
Seawater
Seawater - microbiology
Sequence Analysis, DNA
The Black Sea
Ukraine
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Description
Summary:A slightly creamy, melanogenic, Gram-negative, aerobic bacterium was isolated from seawater sample collected in the Karadag Natural Reserve of the Eastern Crimea, the Black Sea. The novel organism was chemoorganotrophic, had no obligate requirement in NaCl, tolerated to 12% NaCl, grew between 10 and 45 °C, was slightly alkaliphilic, and was not able to degrade starch, gelatin, agar, and Tween 80. 16S rRNA gene sequence-based analyses of the new organism revealed that Oceanimonas doudoroffii ATCC 27123 T, Oceanimonas baumanii ATCC 700832 T, and Oceanisphaera litoralis DSM 15406 T were the closest relatives (similarity around 97%–96%). The G+C content of the DNA of the strain 31-13 T was 55.5 mol%. Phosphatidylethanolamine (49.0%), phosphatidylglycerol (41.8%), and diphosphatidylglycerol (9.2%) were the predominant phospholipids. The major fatty acids were 16:0 (24.1%), 16:1 ω7 (40.3%), and 18:1 ω7 (29.2%). On the basis of the significant differences demonstrated in the phenotypic and chemotaxonomic characteristics, it is suggested that the bacterium be classified as a novel species; the name Oceanimonas smirnovii sp. nov. is proposed. The type strain is 31-13 T (UCM B-11076 T=LMG 22147 T=ATCC BAA-899 T).
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2004.11.002