Production of piglets with sexed semen employing a non-surgical insemination technique

The aim of the present study was to ascertain whether multiparous sows could successfully be inseminated with sexed semen non-surgically. Spermatozoa were stained with Hoechst 33342 and separated flowcytometrically in X- and Y-chromosome bearing sperm populations employing the Beltsville Sperm Sexin...

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Bibliographic Details
Published in:Theriogenology 2005-05, Vol.63 (8), p.2269-2277
Main Authors: Grossfeld, R., Klinc, P., Sieg, B., Rath, D.
Format: Article
Language:English
Subjects:
animal fertility
Animals
artificial insemination
Benzimidazoles
Birth Weight
Female
Flow Cytometry
Fluorescent Dyes
High speed flowcytometry
Insemination, Artificial - methods
Insemination, Artificial - veterinary
Intrauterine insemination
Litter Size
Male
Pig
piglets
Pregnancy
Pregnancy Outcome
pregnancy rate
semen
Sex Determination Analysis - veterinary
sex preselection
Sex Preselection - methods
Sex Preselection - veterinary
sex ratio
Sexing
sows
Sperm Motility
sperm sexing
Spermatozoa
Spermatozoa - physiology
Spermatozoa - ultrastructure
Swine
X Chromosome
Y Chromosome
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Summary:The aim of the present study was to ascertain whether multiparous sows could successfully be inseminated with sexed semen non-surgically. Spermatozoa were stained with Hoechst 33342 and separated flowcytometrically in X- and Y-chromosome bearing sperm populations employing the Beltsville Sperm Sexing Technology (BSST). After weaning, estrus was induced in sows with PMSG and hCG. Animals were inseminated once per estrus non-surgically with a specially designed catheter into the tip of the uterine horn, employing 50 × 10 6 of either sexed or non-sexed spermatozoa diluted in 2 ml Androhep™. Pregnant sows were allowed to go to term. Mean pregnancy rate from inseminations with unsexed spermatozoa was 54.5% whereas inseminations with sexed spermatozoa resulted in 33.3% pregnant sows. All but one piglet born after insemination with sexed semen were of the predicted sex. The sex of those piglets born after inseminations with non-sexed spermatozoa was 61.1% for male and 38.9% for female sex. It is concluded that non-surgically inseminations with flowcytometrically sexed spermatozoa can be conducted successfully.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2004.10.004