Real-Time Detection of Hyperosmotic Stress Response in Optically Trapped Single Yeast Cells Using Raman Microspectroscopy

Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exer...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-04, Vol.77 (8), p.2564-2568
Main Authors: Singh, Gajendra P, Creely, Caitriona M, Volpe, Giovanni, Grötsch, Helga, Petrov, Dmitri
Format: Article
Language:English
Subjects:
Analytical chemistry
Cellular biology
Chemistry
Ethanol - analysis
Exact sciences and technology
Glycerol - analysis
Hot Temperature
Optical Tweezers
Optics and Photonics
Osmotic Pressure
Saccharomyces cerevisiae
Saccharomyces cerevisiae - physiology
Spectrometric and optical methods
Spectrum Analysis, Raman - methods
Stress
Yeast
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Summary:Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substancesglycerol and ethanolcan be monitored in real time in a single cell.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac048359j