Inhibition of the growth of Paenibacillus larvae, the causal agent of American foulbrood of honeybees, by selected strains of aerobic spore-forming bacteria isolated from apiarian sources

The bacterium Paenibacillus larvae, the causative agent of American foulbrood disease of honeybee larvae, occurs throughout the world and is found in many beekeeping areas of Argentina. The potential as biocontrol agents of antagonic aerobic spore-forming bacteria isolated from honey samples and oth...

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Bibliographic Details
Published in:Journal of invertebrate pathology 2006-03, Vol.91 (3), p.141-146
Main Authors: Alippi, Adriana M., Reynaldi, Francisco J.
Format: Article
Language:English
Subjects:
American foulbrood
Animal productions
Animals
Apiculture
Apis mellifera
Bacillus - classification
Bacillus - growth & development
Bacillus cereus
Bacillus cereus - growth & development
Bacillus licheniformis
Bacillus megaterium
Bacillus pumilus
Bacillus subtilis
Bacteria, Aerobic - physiology
Bacterial antagonists
Bacterial Typing Techniques
Bacteriological Techniques
Bees - microbiology
Biocontrol
Biological and medical sciences
Brevibacillus laterosporus
Fundamental and applied biological sciences. Psychology
Honey - microbiology
Honeybees
Insecta
Invertebrates
Larva - growth & development
Larva - microbiology
Paenibacillus larvae
Pest Control, Biological
Pumilus
Spores, Bacterial - physiology
Terrestrial animal productions
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Summary:The bacterium Paenibacillus larvae, the causative agent of American foulbrood disease of honeybee larvae, occurs throughout the world and is found in many beekeeping areas of Argentina. The potential as biocontrol agents of antagonic aerobic spore-forming bacteria isolated from honey samples and other apiarian sources were evaluated. Each isolate was screened against one strain of Paenibacillus larvae (ATCC 9545) by using a perpendicular streak technique. Ten randomly selected bacterial strains from the group that showed the best antagonistic effect to P. larvae ATCC 9545 were selected for further study. These were identified as Bacillus subtilis (m351), B. pumilus (m350), B. licheniformis (m347), B. cereus (mv33), B. cereus (m387), B. cereus (m6c), B. megaterium (m404), Brevibacillus laterosporus (BLAT169), B. laterosporus (BLAT170), and B. laterosporus (BLAT171). The antagonistic strains were tested against 17 P. larvae strains from different geographical origins by means of a spot test in wells. The analysis of variance and posterior comparison of means by Tukey method ( P < 0.01) showed that the best antagonists were B. megaterium (m404), B. licheniformis (m347), B. cereus (m6c), B. cereus (mv33), and B. cereus (m387).
ISSN:0022-2011
1096-0805
DOI:10.1016/j.jip.2005.12.002