Human herpesvirus 6 (HHV-6) U94/REP protein inhibits betaherpesvirus replication

Human herpesvirus 6 (HHV-6) is the only human herpesvirus encoding U94/rep, homologue to the parvovirus non-structural gene rep68/78. Results to date suggest that HHV-6 U94/rep might regulate viral gene expression and have a role in viral latency. To determine the effect of U94/REP upon viral replic...

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Published in:Virology (New York, N.Y.) N.Y.), 2006-03, Vol.346 (2), p.402-414
Main Authors: Caselli, Elisabetta, Bracci, Arianna, Galvan, Monica, Boni, Michela, Rotola, Antonella, Bergamini, Carlo, Cermelli, Claudio, Dal Monte, Paola, Gompels, Ursula A., Cassai, Enzo, Di Luca, Dario
Format: Article
Language:English
Subjects:
Betaherpesvirinae - physiology
Betaherpesvirus inhibition
Cell Line
Cell Nucleus - chemistry
Cytomegalovirus - physiology
Herpes simplex virus
Herpesvirus 6, Human - physiology
Herpesvirus 7, Human - physiology
HHV-6
Human cytomegalovirus
Human herpesvirus
Human herpesvirus 6
Humans
Parvovirus
Protein Transport
Simplexvirus - physiology
U94/rep gene
Viral Proteins - isolation & purification
Viral Proteins - metabolism
Viral Proteins - physiology
Virus Replication
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Summary:Human herpesvirus 6 (HHV-6) is the only human herpesvirus encoding U94/rep, homologue to the parvovirus non-structural gene rep68/78. Results to date suggest that HHV-6 U94/rep might regulate viral gene expression and have a role in viral latency. To determine the effect of U94/REP upon viral replication, the protein was produced. The purified U94/REP retained the characteristic immunological features. It was internalized and localized in the nucleus of human cells, showing marked inhibitory activity on the replication of HHV-6 (both variants A and B). The effect of U94/REP was dose-dependent and sensitive to treatment with single-stranded but not double-stranded DNA. U94/REP inhibited the replication of other betaherpesviruses, HHV-7 and human cytomegalovirus, but had no effect on herpes simplex virus. These results confirm the action of U94/rep latency gene in the regulation of HHV-6 replication with implications for co-reactivations and latency of human betaherpesviruses.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2005.11.018