Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein

Summary Recently we cloned the Hanp1 cDNA that encodes a histone H1‐like haploid germ cell‐specific nuclear protein in the mouse. Homozygous Hanp1 mutant male mice were infertile, while females were fertile. Although a substantial number of sperm were recovered from the epididymis, their shape and f...

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Published in:International journal of andrology 2006-04, Vol.29 (2), p.353-359
Main Authors: TANAKA, HIROMITSU, MATSUOKA, YASUHIRO, ONISHI, MASAYOSHI, KITAMURA, KOUICHI, MIYAGAWA, YASUSHI, NISHIMURA, HIROMI, TSUJIMURA, AKIRA, OKUYAMA, AKIHIKO, NISHIMUNE, YOSHITAKE
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Case-Control Studies
Cell Line
Cell Nucleus - metabolism
chromatin
Fundamental and applied biological sciences. Psychology
Gene Expression
Gynecology. Andrology. Obstetrics
histone
Histones - chemistry
Histones - genetics
Histones - metabolism
Humans
Infertility, Male - genetics
Male
Male genital diseases
Mammalian male genital system
Medical sciences
Molecular Sequence Data
nucleus
Polymorphism, Single Nucleotide
RNA, Messenger - metabolism
Sequence Homology
single-nucleotide polymorphisms
sperm
spermiogenesis
testis
Testis - metabolism
Tissue Distribution
Transcription, Genetic
Transfection
Vertebrates: reproduction
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Summary:Summary Recently we cloned the Hanp1 cDNA that encodes a histone H1‐like haploid germ cell‐specific nuclear protein in the mouse. Homozygous Hanp1 mutant male mice were infertile, while females were fertile. Although a substantial number of sperm were recovered from the epididymis, their shape and function were abnormal. Hanp1 protein is essential for nuclear formation in functional spermatozoa, and is specifically involved in the replacement of histones with protamines during spermiogenesis. To investigate the roles of human HANP1 (h‐HANP1) and its relation to male infertility, we isolated h‐HANP1 cDNA from a human cDNA plasmid library using mouse Hanp1 cDNA as a probe. h‐HANP1 is expressed in the testes and its genomic construct also intronless as mouse Hanp1. We found that the h‐HANP1 coding region have 5 single‐nucleotide polymorphisms in Japanese men.
ISSN:0105-6263
1365-2605
DOI:10.1111/j.1365-2605.2005.00600.x