Gold Nanoparticle-Enhanced Secondary Ion Mass Spectrometry Imaging of Peptides on Self-Assembled Monolayers

We demonstrate the use of gold nanoparticles (AuNPs) to enhance the secondary ion emission of peptides in time-of-flight secondary ion mass spectrometry (TOF-SIMS). The signal intensity of peptides adsorbed onto AuNPs was significantly increased when compared to that of self-assembled monolayers (SA...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2006-03, Vol.78 (6), p.1913-1920
Main Authors: Kim, Young-Pil, Oh, Eunkeu, Hong, Mi-Young, Lee, Dohoon, Han, Min-Kyu, Shon, Hyun Kyong, Moon, Dae Won, Kim, Hak-Sung, Lee, Tae Geol
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Exact sciences and technology
Gold
Gold - chemistry
Ions
Mass spectrometry
Membranes, Artificial
Nanoparticles
Nanoparticles - chemistry
Particle Size
Peptides
Peptides - analysis
Scientific imaging
Sensitivity and Specificity
Spectrometric and optical methods
Spectrometry, Mass, Secondary Ion - methods
Surface Plasmon Resonance
Surface Properties
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We demonstrate the use of gold nanoparticles (AuNPs) to enhance the secondary ion emission of peptides in time-of-flight secondary ion mass spectrometry (TOF-SIMS). The signal intensity of peptides adsorbed onto AuNPs was significantly increased when compared to that of self-assembled monolayers (SAMs). This gold nanoparticle-enhanced SIMS, termed NE-SIMS, enabled the sensitive detection of subtle modifications of peptides, such as phosphorylation. From a quantitative analysis of the amounts of adsorbed peptides and AuNPs on SAMs using quartz crystal microbalance and surface plasmon resonance spectroscopy, the ratio of peptide molecule to AuNP on amine-SAMs was revealed to be 18−19:1. When considering the ratio of peptide to matrix (1:103−106) employed in a matrix-enhanced SIMS, the use of AuNPs gave rise to a significantly increased secondary ion emission of peptides. Peptides were adsorbed onto patterned AuNPs on SAMs using a microfluidic system, and well-contrasted molecular ion images were obtained. NE-SIMS is expected to be applied to a chip-based analysis of modification of biomolecules in a label-free manner.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac051500j