Detection of the frequency, antimicrobial susceptibility, and genotypic discrimination of Aeromonas strains isolated from municipally treated tap water samples by cultivation and AP-PCR

• Published in: International journal of food microbiology 2006-04, Vol.107 (3), p.310-314
• Main Authors: Emekdas, Gurol, Aslan, Gonul, Tezcan, Seda, Serin, Mehmet Sami, Yildiz, Cilem, Ozturhan, Hakan, Durmaz, Riza
• Format: Article
• Language: English
• Subjects: Aeromonas; Aeromonas - classification; Aeromonas - drug effects; Aeromonas - genetics; Aeromonas - isolation & purification; Aeromonas hydrophila; Anti-Bacterial Agents - pharmacology; antibiotic resistance; AP-PCR; Biological and medical sciences; Drug Resistance, Bacterial; food contamination; Food industries; Food microbiology; food pathogens; Fundamental and applied biological sciences. Psychology; General aspects; Genotype; isolation; Methods of analysis, processing and quality control, regulation, standards; Microbial Sensitivity Tests; Phylogeny; Polymerase Chain Reaction; serotypes; tap water; Vibrio fluvialis; Water born infection; Water Microbiology; water pollution; water supply; Water Supply - analysis; waterborne diseases
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2005.10.012

The frequency, antibiotic susceptibility, and genotypic discrimination of Aeromonas strains isolated from municipally treated drinking tap water distribution systems were investigated in this study. We have analyzed 148 tap water samples collected from 8 different locations by bacterial cultivation and arbitrarily primed polymerase chain reaction (AP-PCR). Gram negative, hemolytic, oxidase (+) and catalase (+) bacterial colonies were applied to the study. Identification of bacterial colonies was done by conventional biochemical method and API ID 20E panel (BioMerieux-France). Molecular epidemiological discrimination of the isolates was done by AP-PCR. Aeromonas spp. was detected in 6 of 148 (4%) tap water samples from 8 different locations. Five isolates were identified as Aeromonas hydrophila and one isolate was identified as Vibrio fluvialis by conventional biochemical method. These data were also confirmed by API 20E panel. One of 6 isolates was resistant to gentamicin, 2 of 6 isolates were resistant to trimethoprim/sulfamethoxazole, 4 of 6 isolates were resistant to ampicillin and ampicillin-sulbactam and all of 6 isolates were resistant to cephalothin. All isolates were found to be susceptible to amikacin, aztreonam, ceftazidime, ceftriaxone, ciprofloxacin. All 6 strains of Aeromonas were discriminated by AP-PCR and were determined that all isolates were from different genotypic sources. Although the frequency of the isolates was under the standard limits, the results indicate that hemolytic A. hydrophila are present in municipally treated tap water samples in Mersin City. While all strains were genotypically distinct, all of them were resistant to first generation beta lactam antibiotics tested in this study.