Selection of Streptavidin Binders from a DNA-Encoded Chemical Library

DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assembling chemical libraries for the identification of high-affinity binders to protein targets. We have constructed a library of 477 chemical compounds, coupled to 48mer-oligonucleotides, each conta...

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Bibliographic Details
Published in:Bioconjugate chemistry 2006-03, Vol.17 (2), p.366-370
Main Authors: Dumelin, Christoph E, Scheuermann, Jörg, Melkko, Samu, Neri, Dario
Format: Article
Language:English
Subjects:
Animals
Chemical bonds
Chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
Gene Library
Humans
Molecular Structure
Protein Binding
Proteins
Streptavidin - chemistry
Streptavidin - metabolism
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Summary:DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assembling chemical libraries for the identification of high-affinity binders to protein targets. We have constructed a library of 477 chemical compounds, coupled to 48mer-oligonucleotides, each containing a unique six-base sequence serving as “bar-code” for the identification of the chemical moiety. The functionality of the library was confirmed by selection and amplification of both high- and low-affinity binding molecules specific to streptavidin.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc050282y