Investigation of methaemoglobin reduction by extracellular NADH in mammalian erythrocytes

The effect of extracellular NADH on the rate of reduction of nitrite-induced methaemoglobin in erythrocytes from man, cattle, dog, horse, grey kangaroo, pig and sheep was investigated. Extracellular NADH was found to enhance the rate of methaemoglobin reduction in man, dog, pig and kangaroo erythroc...

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Published in:The international journal of biochemistry & cell biology 2005-07, Vol.37 (7), p.1438-1445
Main Authors: Kennett, Eleanor C., Ogawa, Eri, Agar, Nihal S., Godwin, Ian R., Bubb, William A., Kuchel, Philip W.
Format: Article
Language:English
Subjects:
Animals
Coloring Agents
Erythrocytes - physiology
Extracellular Fluid - metabolism
Humans
Mammalian erythrocytes
Mammals
Methaemoglobin
Methemoglobin - metabolism
Methylene Blue
NAD - physiology
NADH
Oxidation-Reduction
PMOR
Species Specificity
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Summary:The effect of extracellular NADH on the rate of reduction of nitrite-induced methaemoglobin in erythrocytes from man, cattle, dog, horse, grey kangaroo, pig and sheep was investigated. Extracellular NADH was found to enhance the rate of methaemoglobin reduction in man, dog, pig and kangaroo erythrocytes, but had essentially no effect on the rate of methaemoglobin reduction in erythrocytes from cattle, horse and sheep. In erythrocytes of those animals affected by extracellular NADH the rate of reduction of metHb in the presence of NADH was the same or greater than that observed in the presence of nutrients such as glucose and inosine. The combination of nutrient and NADH produced a more profound increase in the rate of methaemoglobin reduction. The rate of methaemoglobin reduction in all cases was significantly less than that observed with methylene blue, the standard treatment of methaemoglobinaemia. Extracellular NADH was found to indirectly increase the intracellular NADH concentration through displacement of the pseudo-equilibrium of the intracellular LDH reaction and relied upon the presence of sufficient LDH activity released into the extracellular medium through haemolysis. The lack of response of cattle, horse and sheep RBCs to extracellular NADH was found to derive mainly from their low extracellular LDH activity, but also correlated with their lower NADH-methaemoglobin reductase activity compared to the other species.
ISSN:1357-2725
1878-5875
DOI:10.1016/j.biocel.2005.02.001