Development of a nested PCR and its internal control for the detection of African swine fever virus (ASFV) in Ornithodoros erraticus

A nested PCR assay, with an internal control, was developed to detect African swine fever virus (ASFV) DNA in Ornithodoros erraticus. The assay revealed a better analytical sensitivity than virus isolation and the OIE PCR protocol. All ticks collected from the field, which were positive by virus iso...

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Bibliographic Details
Published in:Archives of virology 2006-04, Vol.151 (4), p.819-826
Main Authors: BASTO, A. P, PORTUGAL, R. S, NIX, R. J, CARTAXEIRO, C, BOINAS, F, DIXON, L. K, LEITAO, A, MARTINS, C
Format: Article
Language:English
Subjects:
African swine fever
African Swine Fever - virology
African swine fever virus
African Swine Fever Virus - genetics
African Swine Fever Virus - isolation & purification
Biological and medical sciences
Cloning
Disease
Disease Reservoirs - veterinary
Disease Reservoirs - virology
DNA Primers
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Ixodidae
Microbiology
Miscellaneous
Ornithodoros
Ornithodoros - virology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Thermal cycling
Virology
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Summary:A nested PCR assay, with an internal control, was developed to detect African swine fever virus (ASFV) DNA in Ornithodoros erraticus. The assay revealed a better analytical sensitivity than virus isolation and the OIE PCR protocol. All ticks collected from the field, which were positive by virus isolation, were also positive by PCR. Viral DNA was detected in a further 19 out of 60 ticks from which no virus was isolated. Our results show that this assay is reliable and can easily be used to screen large tick populations collected in the field for the presence of ASFV.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-005-0654-2