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N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay

The title compound is a single step cleavage fluorogenic substrate and has significantly higher enzyme turnover rate and sensitivity than existing fluorogenic substrates for detecting caspase-3 activity both in solution and living cells. A novel caspase-3 substrate N-Ac-DEVD-N′-MC-R110, which is a f...

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Published in:Bioorganic & medicinal chemistry letters 2005-05, Vol.15 (9), p.2335-2338
Main Authors: Wang, Zhi-Qiang, Liao, Jinfang, Diwu, Zhenjun
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description The title compound is a single step cleavage fluorogenic substrate and has significantly higher enzyme turnover rate and sensitivity than existing fluorogenic substrates for detecting caspase-3 activity both in solution and living cells. A novel caspase-3 substrate N-Ac-DEVD-N′-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates.
doi_str_mv 10.1016/j.bmcl.2005.02.081
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source ScienceDirect Journals
subjects Ageing, cell death
Analytical, structural and metabolic biochemistry
Apoptosis
Biological and medical sciences
Caspase
Caspase 3
Caspases - metabolism
Cell physiology
Enzymes and enzyme inhibitors
Fluorescent
Fluorogenic substrates
Fundamental and applied biological sciences. Psychology
HTS
Humans
Hydrolases
Indicators and Reagents
Kinetics
Models, Molecular
Molecular and cellular biology
Molecular Structure
Morpholines - chemical synthesis
Morpholines - chemistry
Morpholines - pharmacology
Oligopeptides - chemical synthesis
Oligopeptides - chemistry
Oligopeptides - pharmacology
Recombinant Proteins - metabolism
Rhodamines - chemical synthesis
Rhodamines - chemistry
Rhodamines - pharmacology
Substrate Specificity
Synthesis
title N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay
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