Loading…
N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay
The title compound is a single step cleavage fluorogenic substrate and has significantly higher enzyme turnover rate and sensitivity than existing fluorogenic substrates for detecting caspase-3 activity both in solution and living cells. A novel caspase-3 substrate N-Ac-DEVD-N′-MC-R110, which is a f...
Saved in:
Published in: | Bioorganic & medicinal chemistry letters 2005-05, Vol.15 (9), p.2335-2338 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c450t-582986d2ee574159ba850f4b05a2fa49211c702d7a940941f02c8a3b7dda717e3 |
---|---|
cites | cdi_FETCH-LOGICAL-c450t-582986d2ee574159ba850f4b05a2fa49211c702d7a940941f02c8a3b7dda717e3 |
container_end_page | 2338 |
container_issue | 9 |
container_start_page | 2335 |
container_title | Bioorganic & medicinal chemistry letters |
container_volume | 15 |
creator | Wang, Zhi-Qiang Liao, Jinfang Diwu, Zhenjun |
description | The title compound is a single step cleavage fluorogenic substrate and has significantly higher enzyme turnover rate and sensitivity than existing fluorogenic substrates for detecting caspase-3 activity both in solution and living cells.
A novel caspase-3 substrate N-Ac-DEVD-N′-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates. |
doi_str_mv | 10.1016/j.bmcl.2005.02.081 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67753300</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960894X0500274X</els_id><sourcerecordid>67753300</sourcerecordid><originalsourceid>FETCH-LOGICAL-c450t-582986d2ee574159ba850f4b05a2fa49211c702d7a940941f02c8a3b7dda717e3</originalsourceid><addsrcrecordid>eNp9kM1u1DAUhS1ERYeWF2CBsoGdw7VjxwliU7XlR6raTUHsrBvHoR4lcfBNKs2OZ-KReBIympG6Y3Wlq-8cHX2MvRaQCxDl-23eDK7PJYDOQeZQiWdsI1SpeKFAP2cbqEvgVa1-nLKXRFsAoUCpF-xU6KowhYQNo1t-df39it_-_f2HDzFND7EPo3eYmjjuep4eYovD-smEgA_ZGB99nzmkCcnzIuv6Jab404_BZbQ0NCecPWVdTJnzfc-bFWsznOI0RwqUIRHuztlJhz35V8d7xr59ur6__MJv7j5_vby44U5pmLmuZF2VrfReGyV03WCloVMNaJQdqloK4QzI1mCtoFaiA-kqLBrTtmiE8cUZe3fonVL8tXia7RBoPwtHHxeypTG6KABWUB5AlyJR8p2dUhgw7awAu1dtt3av2u5VW5B2Vb2G3hzbl2bw7VPk6HYF3h4BJId9l3B0gZ640ghZmnrlPh44v7p4DD5ZcsGPzrcheTfbNob_7fgHFludpw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67753300</pqid></control><display><type>article</type><title>N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay</title><source>ScienceDirect Journals</source><creator>Wang, Zhi-Qiang ; Liao, Jinfang ; Diwu, Zhenjun</creator><creatorcontrib>Wang, Zhi-Qiang ; Liao, Jinfang ; Diwu, Zhenjun</creatorcontrib><description>The title compound is a single step cleavage fluorogenic substrate and has significantly higher enzyme turnover rate and sensitivity than existing fluorogenic substrates for detecting caspase-3 activity both in solution and living cells.
A novel caspase-3 substrate N-Ac-DEVD-N′-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates.</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/j.bmcl.2005.02.081</identifier><identifier>PMID: 15837320</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Ageing, cell death ; Analytical, structural and metabolic biochemistry ; Apoptosis ; Biological and medical sciences ; Caspase ; Caspase 3 ; Caspases - metabolism ; Cell physiology ; Enzymes and enzyme inhibitors ; Fluorescent ; Fluorogenic substrates ; Fundamental and applied biological sciences. Psychology ; HTS ; Humans ; Hydrolases ; Indicators and Reagents ; Kinetics ; Models, Molecular ; Molecular and cellular biology ; Molecular Structure ; Morpholines - chemical synthesis ; Morpholines - chemistry ; Morpholines - pharmacology ; Oligopeptides - chemical synthesis ; Oligopeptides - chemistry ; Oligopeptides - pharmacology ; Recombinant Proteins - metabolism ; Rhodamines - chemical synthesis ; Rhodamines - chemistry ; Rhodamines - pharmacology ; Substrate Specificity ; Synthesis</subject><ispartof>Bioorganic & medicinal chemistry letters, 2005-05, Vol.15 (9), p.2335-2338</ispartof><rights>2005 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-582986d2ee574159ba850f4b05a2fa49211c702d7a940941f02c8a3b7dda717e3</citedby><cites>FETCH-LOGICAL-c450t-582986d2ee574159ba850f4b05a2fa49211c702d7a940941f02c8a3b7dda717e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16712679$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15837320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Zhi-Qiang</creatorcontrib><creatorcontrib>Liao, Jinfang</creatorcontrib><creatorcontrib>Diwu, Zhenjun</creatorcontrib><title>N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>The title compound is a single step cleavage fluorogenic substrate and has significantly higher enzyme turnover rate and sensitivity than existing fluorogenic substrates for detecting caspase-3 activity both in solution and living cells.
A novel caspase-3 substrate N-Ac-DEVD-N′-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates.</description><subject>Ageing, cell death</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Caspase</subject><subject>Caspase 3</subject><subject>Caspases - metabolism</subject><subject>Cell physiology</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fluorescent</subject><subject>Fluorogenic substrates</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HTS</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Indicators and Reagents</subject><subject>Kinetics</subject><subject>Models, Molecular</subject><subject>Molecular and cellular biology</subject><subject>Molecular Structure</subject><subject>Morpholines - chemical synthesis</subject><subject>Morpholines - chemistry</subject><subject>Morpholines - pharmacology</subject><subject>Oligopeptides - chemical synthesis</subject><subject>Oligopeptides - chemistry</subject><subject>Oligopeptides - pharmacology</subject><subject>Recombinant Proteins - metabolism</subject><subject>Rhodamines - chemical synthesis</subject><subject>Rhodamines - chemistry</subject><subject>Rhodamines - pharmacology</subject><subject>Substrate Specificity</subject><subject>Synthesis</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp9kM1u1DAUhS1ERYeWF2CBsoGdw7VjxwliU7XlR6raTUHsrBvHoR4lcfBNKs2OZ-KReBIympG6Y3Wlq-8cHX2MvRaQCxDl-23eDK7PJYDOQeZQiWdsI1SpeKFAP2cbqEvgVa1-nLKXRFsAoUCpF-xU6KowhYQNo1t-df39it_-_f2HDzFND7EPo3eYmjjuep4eYovD-smEgA_ZGB99nzmkCcnzIuv6Jab404_BZbQ0NCecPWVdTJnzfc-bFWsznOI0RwqUIRHuztlJhz35V8d7xr59ur6__MJv7j5_vby44U5pmLmuZF2VrfReGyV03WCloVMNaJQdqloK4QzI1mCtoFaiA-kqLBrTtmiE8cUZe3fonVL8tXia7RBoPwtHHxeypTG6KABWUB5AlyJR8p2dUhgw7awAu1dtt3av2u5VW5B2Vb2G3hzbl2bw7VPk6HYF3h4BJId9l3B0gZ640ghZmnrlPh44v7p4DD5ZcsGPzrcheTfbNob_7fgHFludpw</recordid><startdate>20050502</startdate><enddate>20050502</enddate><creator>Wang, Zhi-Qiang</creator><creator>Liao, Jinfang</creator><creator>Diwu, Zhenjun</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050502</creationdate><title>N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay</title><author>Wang, Zhi-Qiang ; Liao, Jinfang ; Diwu, Zhenjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-582986d2ee574159ba850f4b05a2fa49211c702d7a940941f02c8a3b7dda717e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Ageing, cell death</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Caspase</topic><topic>Caspase 3</topic><topic>Caspases - metabolism</topic><topic>Cell physiology</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fluorescent</topic><topic>Fluorogenic substrates</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HTS</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Indicators and Reagents</topic><topic>Kinetics</topic><topic>Models, Molecular</topic><topic>Molecular and cellular biology</topic><topic>Molecular Structure</topic><topic>Morpholines - chemical synthesis</topic><topic>Morpholines - chemistry</topic><topic>Morpholines - pharmacology</topic><topic>Oligopeptides - chemical synthesis</topic><topic>Oligopeptides - chemistry</topic><topic>Oligopeptides - pharmacology</topic><topic>Recombinant Proteins - metabolism</topic><topic>Rhodamines - chemical synthesis</topic><topic>Rhodamines - chemistry</topic><topic>Rhodamines - pharmacology</topic><topic>Substrate Specificity</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Zhi-Qiang</creatorcontrib><creatorcontrib>Liao, Jinfang</creatorcontrib><creatorcontrib>Diwu, Zhenjun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zhi-Qiang</au><au>Liao, Jinfang</au><au>Diwu, Zhenjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2005-05-02</date><risdate>2005</risdate><volume>15</volume><issue>9</issue><spage>2335</spage><epage>2338</epage><pages>2335-2338</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>The title compound is a single step cleavage fluorogenic substrate and has significantly higher enzyme turnover rate and sensitivity than existing fluorogenic substrates for detecting caspase-3 activity both in solution and living cells.
A novel caspase-3 substrate N-Ac-DEVD-N′-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15837320</pmid><doi>10.1016/j.bmcl.2005.02.081</doi><tpages>4</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0960-894X |
ispartof | Bioorganic & medicinal chemistry letters, 2005-05, Vol.15 (9), p.2335-2338 |
issn | 0960-894X 1464-3405 |
language | eng |
recordid | cdi_proquest_miscellaneous_67753300 |
source | ScienceDirect Journals |
subjects | Ageing, cell death Analytical, structural and metabolic biochemistry Apoptosis Biological and medical sciences Caspase Caspase 3 Caspases - metabolism Cell physiology Enzymes and enzyme inhibitors Fluorescent Fluorogenic substrates Fundamental and applied biological sciences. Psychology HTS Humans Hydrolases Indicators and Reagents Kinetics Models, Molecular Molecular and cellular biology Molecular Structure Morpholines - chemical synthesis Morpholines - chemistry Morpholines - pharmacology Oligopeptides - chemical synthesis Oligopeptides - chemistry Oligopeptides - pharmacology Recombinant Proteins - metabolism Rhodamines - chemical synthesis Rhodamines - chemistry Rhodamines - pharmacology Substrate Specificity Synthesis |
title | N-DEVD-N′-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T11%3A40%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=N-DEVD-N%E2%80%B2-morpholinecarbonyl-rhodamine%20110:%20novel%20caspase-3%20fluorogenic%20substrates%20for%20cell-based%20apoptosis%20assay&rft.jtitle=Bioorganic%20&%20medicinal%20chemistry%20letters&rft.au=Wang,%20Zhi-Qiang&rft.date=2005-05-02&rft.volume=15&rft.issue=9&rft.spage=2335&rft.epage=2338&rft.pages=2335-2338&rft.issn=0960-894X&rft.eissn=1464-3405&rft_id=info:doi/10.1016/j.bmcl.2005.02.081&rft_dat=%3Cproquest_cross%3E67753300%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c450t-582986d2ee574159ba850f4b05a2fa49211c702d7a940941f02c8a3b7dda717e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=67753300&rft_id=info:pmid/15837320&rfr_iscdi=true |