Differentiation of Mycoplasma gallisepticum Strains Using Amplified Fragment Length Polymorphism and Other DNA-Based Typing Methods

Amplified fragment length polymorphism (AFLP) was used to type 34 strains of Mycoplasma gallisepticum (MG) including vaccine strains ts-11, 6/85, and F. Using AFLP, a total of 10 groups, with 30 distinguishable AFLP typing profiles, were generated in the analysis. The AFLP method was able to identif...

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Bibliographic Details
Published in:Avian diseases 2005-03, Vol.49 (1), p.43-49
Main Authors: Hong, Yang, García, Maricarmen, Levisohn, Sharon, Savelkoul, Paul, Leiting, Victoria, Lysnyansky, Inna, Ley, David H, Kleven, Stanley H
Format: Article
Language:English
Subjects:
amplified fragment length polymorphism
animal pathogenic bacteria
bacterial proteins
Bacterial Proteins - genetics
Bacterial Typing Techniques - methods
Base Sequence
DNA
DNA fingerprinting
DNA Primers
Gels
Genetic polymorphism
Genetic Variation
microbial genetics
Molecular Sequence Data
Molecular typing
Mycoplasma
Mycoplasma gallisepticum
Mycoplasma gallisepticum - genetics
mycoplasmosis
Nucleic Acid Amplification Techniques
nucleotide sequences
Nucleotides
pathogen identification
Polymerase chain reaction
Polymorphism, Restriction Fragment Length
poultry diseases
random amplified polymorphic DNA
Random Amplified Polymorphic DNA Technique
Regular s
Reproducibility of Results
sequence analysis
Sequence Analysis, DNA
Sequencing
Species Specificity
strain differences
strain identification
strains
Vaccination
vaccines
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Summary:Amplified fragment length polymorphism (AFLP) was used to type 34 strains of Mycoplasma gallisepticum (MG) including vaccine strains ts-11, 6/85, and F. Using AFLP, a total of 10 groups, with 30 distinguishable AFLP typing profiles, were generated in the analysis. The AFLP method was able to identify and differentiate both MG field strains from recent outbreaks and those that were epidemiologically related. The AFLP procedure will provide assistance in identifying the sources of mycoplasma infections. Vaccine strains were also differentiated from other field strains, which will be useful in the evaluation of vaccination programs. The AFLP discrimination potential was compared to other molecular typing techniques such as gene-targeted typing by DNA sequence analysis of the MG cytadhesin-like protein encoding gene, mgc2, and random amplified polymorphic DNA assay on the same MG isolates. The three assays correlated well with one another, with AFLP analysis having a much higher discriminatory power and reproducibility.
ISSN:0005-2086
1938-4351
DOI:10.1637/7254-080504R