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Continuous Fabrication of Biocatalyst Immobilized Microparticles Using Photopolymerization and Immiscible Liquids in Microfluidic Systems

We report a novel technique for manufacturing polymeric microparticles containing biocatalysts by the behavior of immiscible liquids in microfluidic systems and in situ photopolymerization. The approach utilizes a UV-polymerizable hydrogel/enzyme solution and an immiscible oil solution. The oil and...

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Bibliographic Details
Published in:Langmuir 2005-04, Vol.21 (9), p.3738-3741
Main Authors: Jeong, Won Je, Kim, Jeong Yun, Choo, Jaebum, Lee, Eun Kyu, Han, Chang Soo, Beebe, David J, Seong, Gi Hun, Lee, Sang Hoon
Format: Article
Language:English
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Summary:We report a novel technique for manufacturing polymeric microparticles containing biocatalysts by the behavior of immiscible liquids in microfluidic systems and in situ photopolymerization. The approach utilizes a UV-polymerizable hydrogel/enzyme solution and an immiscible oil solution. The oil and hydrogel solutions form emulsions in pressure-driven flow in microchannels at high values of the dimensionless capillary number (Ca). The resultant hydrogel droplets are then polymerized in situ via exposure to 365 nm UV light. This technique allows for the generation of monodisperse particles whose size can be controlled by the regulation of flow rates. In addition, both manufacturing microparticles and immobilizing biocatalysts can be performed simultaneously and continuously.
ISSN:0743-7463
1520-5827
DOI:10.1021/la050105l