Loading…

Transgenic mice with pancellular enhanced green fluorescent protein expression in primitive hematopoietic cells and all blood cell progeny

Transgenic mice homogeneously expressing enhanced green fluorescence protein (EGFP) in primitive hematopoietic cells and all blood cell progeny, including erythrocytes and platelets, have not been reported. Given previous data indicating H2Kb promoter activity in murine hematopoietic stem cells (HSC...

Full description

Saved in:
Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2005-05, Vol.42 (1), p.17-22
Main Authors: Dominici, Massimo, Tadjali, Merhdad, Kepes, Steven, Allay, Esther R., Boyd, Kelli, Ney, Paul A., Horwitz, Edwin, Persons, Derek A.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c4321-e59d6d70c45e927a9ad5a72bbdacf3879cc584fa57b94b454b29f6058fd5f83e3
cites cdi_FETCH-LOGICAL-c4321-e59d6d70c45e927a9ad5a72bbdacf3879cc584fa57b94b454b29f6058fd5f83e3
container_end_page 22
container_issue 1
container_start_page 17
container_title Genesis (New York, N.Y. : 2000)
container_volume 42
creator Dominici, Massimo
Tadjali, Merhdad
Kepes, Steven
Allay, Esther R.
Boyd, Kelli
Ney, Paul A.
Horwitz, Edwin
Persons, Derek A.
description Transgenic mice homogeneously expressing enhanced green fluorescence protein (EGFP) in primitive hematopoietic cells and all blood cell progeny, including erythrocytes and platelets, have not been reported. Given previous data indicating H2Kb promoter activity in murine hematopoietic stem cells (HSCs), bone marrow (BM), and lymphocytes, an H2Kb enhancer/promoter EGFP construct was used to generate transgenic mice. These mice demonstrated pancellular EGFP expression in both primitive BM Sca‐1+Lin−Kit+ cells and side population (SP) cells. Additionally, all peripheral blood leukocytes subsets, erythrocytes, and platelets uniformly expressed EGFP strongly. Competitive BM transplantation assays established that transgenic H2Kb‐EGFP HSCs had activity equivalent to wildtype HSCs in their ability to reconstitute hematopoiesis in lethally irradiated mice. In addition, immunohistochemistry revealed EGFP transgene expression in all tissues examined. This transgenic strain should be a useful reagent for both murine hematopoiesis studies and functional studies of specific cell types from particular tissues. genesis 42:17–22, 2005. © 2005 Wiley‐Liss, Inc.
doi_str_mv 10.1002/gene.20121
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67756940</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17840159</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4321-e59d6d70c45e927a9ad5a72bbdacf3879cc584fa57b94b454b29f6058fd5f83e3</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhiMEoqVw4QGQTxyQUmzHjuMjqpYFVAqIIiouluNMugbHTu2Edl-Bp8bpbuEGp_GM_vlmxn9RPCX4mGBMX16Ch2OKCSX3ikPCaV3Kurm4f_fm7OKgeJTSd4wxbyh9WByQHBuM2WHx6zxqnzLBGjRYA-jaThs0am_AudnpiMBvlqxDlxHAo97NIUIy4Cc0xjCB9QhuxlxKNniUszHawU72J6ANDHoKY7AwZfxCTEj7DmnnUOtC6G5rCyYvsH1cPOi1S_BkH4-KL69X5ydvytMP67cnr05LwypKSuCyqzuBDeMgqdBSd1wL2radNn3VCGkMb1ivuWglaxlnLZV9nS_vO943FVRHxfMdN8-9miFNarBpWUR7CHNStRC8lgz_V0hEwzDhMgtf7IQmhpQi9Gr5Ax23imC1WKQWi9StRVn8bE-d2wG6v9K9J1lAdoJr62D7D5Rar85Wd9By12PTBDd_enT8kc-pBFdfz9bq87f3gn36-E7V1W9in68I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17840159</pqid></control><display><type>article</type><title>Transgenic mice with pancellular enhanced green fluorescent protein expression in primitive hematopoietic cells and all blood cell progeny</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Dominici, Massimo ; Tadjali, Merhdad ; Kepes, Steven ; Allay, Esther R. ; Boyd, Kelli ; Ney, Paul A. ; Horwitz, Edwin ; Persons, Derek A.</creator><creatorcontrib>Dominici, Massimo ; Tadjali, Merhdad ; Kepes, Steven ; Allay, Esther R. ; Boyd, Kelli ; Ney, Paul A. ; Horwitz, Edwin ; Persons, Derek A.</creatorcontrib><description>Transgenic mice homogeneously expressing enhanced green fluorescence protein (EGFP) in primitive hematopoietic cells and all blood cell progeny, including erythrocytes and platelets, have not been reported. Given previous data indicating H2Kb promoter activity in murine hematopoietic stem cells (HSCs), bone marrow (BM), and lymphocytes, an H2Kb enhancer/promoter EGFP construct was used to generate transgenic mice. These mice demonstrated pancellular EGFP expression in both primitive BM Sca‐1+Lin−Kit+ cells and side population (SP) cells. Additionally, all peripheral blood leukocytes subsets, erythrocytes, and platelets uniformly expressed EGFP strongly. Competitive BM transplantation assays established that transgenic H2Kb‐EGFP HSCs had activity equivalent to wildtype HSCs in their ability to reconstitute hematopoiesis in lethally irradiated mice. In addition, immunohistochemistry revealed EGFP transgene expression in all tissues examined. This transgenic strain should be a useful reagent for both murine hematopoiesis studies and functional studies of specific cell types from particular tissues. genesis 42:17–22, 2005. © 2005 Wiley‐Liss, Inc.</description><identifier>ISSN: 1526-954X</identifier><identifier>EISSN: 1526-968X</identifier><identifier>DOI: 10.1002/gene.20121</identifier><identifier>PMID: 15828004</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Blood Platelets - physiology ; Bone Marrow Cells ; Bone Marrow Transplantation - veterinary ; EGFP ; Erythrocytes - physiology ; Female ; Green Fluorescent Proteins - biosynthesis ; Green Fluorescent Proteins - genetics ; Hematopoiesis ; Hematopoietic Stem Cells - physiology ; Immunohistochemistry ; Male ; Mice ; Mice, Transgenic ; Promoter Regions, Genetic ; transgenic</subject><ispartof>Genesis (New York, N.Y. : 2000), 2005-05, Vol.42 (1), p.17-22</ispartof><rights>Copyright © 2005 Wiley‐Liss, Inc.</rights><rights>(c) 2005 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4321-e59d6d70c45e927a9ad5a72bbdacf3879cc584fa57b94b454b29f6058fd5f83e3</citedby><cites>FETCH-LOGICAL-c4321-e59d6d70c45e927a9ad5a72bbdacf3879cc584fa57b94b454b29f6058fd5f83e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15828004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dominici, Massimo</creatorcontrib><creatorcontrib>Tadjali, Merhdad</creatorcontrib><creatorcontrib>Kepes, Steven</creatorcontrib><creatorcontrib>Allay, Esther R.</creatorcontrib><creatorcontrib>Boyd, Kelli</creatorcontrib><creatorcontrib>Ney, Paul A.</creatorcontrib><creatorcontrib>Horwitz, Edwin</creatorcontrib><creatorcontrib>Persons, Derek A.</creatorcontrib><title>Transgenic mice with pancellular enhanced green fluorescent protein expression in primitive hematopoietic cells and all blood cell progeny</title><title>Genesis (New York, N.Y. : 2000)</title><addtitle>Genesis</addtitle><description>Transgenic mice homogeneously expressing enhanced green fluorescence protein (EGFP) in primitive hematopoietic cells and all blood cell progeny, including erythrocytes and platelets, have not been reported. Given previous data indicating H2Kb promoter activity in murine hematopoietic stem cells (HSCs), bone marrow (BM), and lymphocytes, an H2Kb enhancer/promoter EGFP construct was used to generate transgenic mice. These mice demonstrated pancellular EGFP expression in both primitive BM Sca‐1+Lin−Kit+ cells and side population (SP) cells. Additionally, all peripheral blood leukocytes subsets, erythrocytes, and platelets uniformly expressed EGFP strongly. Competitive BM transplantation assays established that transgenic H2Kb‐EGFP HSCs had activity equivalent to wildtype HSCs in their ability to reconstitute hematopoiesis in lethally irradiated mice. In addition, immunohistochemistry revealed EGFP transgene expression in all tissues examined. This transgenic strain should be a useful reagent for both murine hematopoiesis studies and functional studies of specific cell types from particular tissues. genesis 42:17–22, 2005. © 2005 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Blood Platelets - physiology</subject><subject>Bone Marrow Cells</subject><subject>Bone Marrow Transplantation - veterinary</subject><subject>EGFP</subject><subject>Erythrocytes - physiology</subject><subject>Female</subject><subject>Green Fluorescent Proteins - biosynthesis</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Hematopoiesis</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Promoter Regions, Genetic</subject><subject>transgenic</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhiMEoqVw4QGQTxyQUmzHjuMjqpYFVAqIIiouluNMugbHTu2Edl-Bp8bpbuEGp_GM_vlmxn9RPCX4mGBMX16Ch2OKCSX3ikPCaV3Kurm4f_fm7OKgeJTSd4wxbyh9WByQHBuM2WHx6zxqnzLBGjRYA-jaThs0am_AudnpiMBvlqxDlxHAo97NIUIy4Cc0xjCB9QhuxlxKNniUszHawU72J6ANDHoKY7AwZfxCTEj7DmnnUOtC6G5rCyYvsH1cPOi1S_BkH4-KL69X5ydvytMP67cnr05LwypKSuCyqzuBDeMgqdBSd1wL2radNn3VCGkMb1ivuWglaxlnLZV9nS_vO943FVRHxfMdN8-9miFNarBpWUR7CHNStRC8lgz_V0hEwzDhMgtf7IQmhpQi9Gr5Ax23imC1WKQWi9StRVn8bE-d2wG6v9K9J1lAdoJr62D7D5Rar85Wd9By12PTBDd_enT8kc-pBFdfz9bq87f3gn36-E7V1W9in68I</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Dominici, Massimo</creator><creator>Tadjali, Merhdad</creator><creator>Kepes, Steven</creator><creator>Allay, Esther R.</creator><creator>Boyd, Kelli</creator><creator>Ney, Paul A.</creator><creator>Horwitz, Edwin</creator><creator>Persons, Derek A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Transgenic mice with pancellular enhanced green fluorescent protein expression in primitive hematopoietic cells and all blood cell progeny</title><author>Dominici, Massimo ; Tadjali, Merhdad ; Kepes, Steven ; Allay, Esther R. ; Boyd, Kelli ; Ney, Paul A. ; Horwitz, Edwin ; Persons, Derek A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4321-e59d6d70c45e927a9ad5a72bbdacf3879cc584fa57b94b454b29f6058fd5f83e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Blood Platelets - physiology</topic><topic>Bone Marrow Cells</topic><topic>Bone Marrow Transplantation - veterinary</topic><topic>EGFP</topic><topic>Erythrocytes - physiology</topic><topic>Female</topic><topic>Green Fluorescent Proteins - biosynthesis</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Hematopoiesis</topic><topic>Hematopoietic Stem Cells - physiology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Promoter Regions, Genetic</topic><topic>transgenic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dominici, Massimo</creatorcontrib><creatorcontrib>Tadjali, Merhdad</creatorcontrib><creatorcontrib>Kepes, Steven</creatorcontrib><creatorcontrib>Allay, Esther R.</creatorcontrib><creatorcontrib>Boyd, Kelli</creatorcontrib><creatorcontrib>Ney, Paul A.</creatorcontrib><creatorcontrib>Horwitz, Edwin</creatorcontrib><creatorcontrib>Persons, Derek A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genesis (New York, N.Y. : 2000)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dominici, Massimo</au><au>Tadjali, Merhdad</au><au>Kepes, Steven</au><au>Allay, Esther R.</au><au>Boyd, Kelli</au><au>Ney, Paul A.</au><au>Horwitz, Edwin</au><au>Persons, Derek A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic mice with pancellular enhanced green fluorescent protein expression in primitive hematopoietic cells and all blood cell progeny</atitle><jtitle>Genesis (New York, N.Y. : 2000)</jtitle><addtitle>Genesis</addtitle><date>2005-05</date><risdate>2005</risdate><volume>42</volume><issue>1</issue><spage>17</spage><epage>22</epage><pages>17-22</pages><issn>1526-954X</issn><eissn>1526-968X</eissn><abstract>Transgenic mice homogeneously expressing enhanced green fluorescence protein (EGFP) in primitive hematopoietic cells and all blood cell progeny, including erythrocytes and platelets, have not been reported. Given previous data indicating H2Kb promoter activity in murine hematopoietic stem cells (HSCs), bone marrow (BM), and lymphocytes, an H2Kb enhancer/promoter EGFP construct was used to generate transgenic mice. These mice demonstrated pancellular EGFP expression in both primitive BM Sca‐1+Lin−Kit+ cells and side population (SP) cells. Additionally, all peripheral blood leukocytes subsets, erythrocytes, and platelets uniformly expressed EGFP strongly. Competitive BM transplantation assays established that transgenic H2Kb‐EGFP HSCs had activity equivalent to wildtype HSCs in their ability to reconstitute hematopoiesis in lethally irradiated mice. In addition, immunohistochemistry revealed EGFP transgene expression in all tissues examined. This transgenic strain should be a useful reagent for both murine hematopoiesis studies and functional studies of specific cell types from particular tissues. genesis 42:17–22, 2005. © 2005 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15828004</pmid><doi>10.1002/gene.20121</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1526-954X
ispartof Genesis (New York, N.Y. : 2000), 2005-05, Vol.42 (1), p.17-22
issn 1526-954X
1526-968X
language eng
recordid cdi_proquest_miscellaneous_67756940
source Wiley-Blackwell Read & Publish Collection
subjects Animals
Blood Platelets - physiology
Bone Marrow Cells
Bone Marrow Transplantation - veterinary
EGFP
Erythrocytes - physiology
Female
Green Fluorescent Proteins - biosynthesis
Green Fluorescent Proteins - genetics
Hematopoiesis
Hematopoietic Stem Cells - physiology
Immunohistochemistry
Male
Mice
Mice, Transgenic
Promoter Regions, Genetic
transgenic
title Transgenic mice with pancellular enhanced green fluorescent protein expression in primitive hematopoietic cells and all blood cell progeny
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T17%3A21%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transgenic%20mice%20with%20pancellular%20enhanced%20green%20fluorescent%20protein%20expression%20in%20primitive%20hematopoietic%20cells%20and%20all%20blood%20cell%20progeny&rft.jtitle=Genesis%20(New%20York,%20N.Y.%20:%202000)&rft.au=Dominici,%20Massimo&rft.date=2005-05&rft.volume=42&rft.issue=1&rft.spage=17&rft.epage=22&rft.pages=17-22&rft.issn=1526-954X&rft.eissn=1526-968X&rft_id=info:doi/10.1002/gene.20121&rft_dat=%3Cproquest_cross%3E17840159%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4321-e59d6d70c45e927a9ad5a72bbdacf3879cc584fa57b94b454b29f6058fd5f83e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17840159&rft_id=info:pmid/15828004&rfr_iscdi=true