Efficient in-depth trapping with an oil-immersion objective lens

Maximum trapping efficiency in optical tweezers occurs close to the coverslip because spherical aberration owing to a mismatch in the refractive indices of the specimen (water) and the immersion oil dramatically decreases the trap efficiency as the trap depth increases. Measuring the axial trap effi...

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Bibliographic Details
Published in:Optics letters 2006-03, Vol.31 (6), p.766-768
Main Authors: REIHANI, S. Nader S, CHARSOOGHI, Mohammad A, KHALESIFARD, Hamid R, GOLESTANIAN, Ramin
Format: Article
Language:English
Subjects:
Exact sciences and technology
Fundamental areas of phenomenology (including applications)
Mechanical effects of light on atoms, molecules, electrons, and ions
Optics
Physics
Quantum optics
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Summary:Maximum trapping efficiency in optical tweezers occurs close to the coverslip because spherical aberration owing to a mismatch in the refractive indices of the specimen (water) and the immersion oil dramatically decreases the trap efficiency as the trap depth increases. Measuring the axial trap efficiency at various tube lengths by use of an oil-immersion objective has shown that such an aberration can be balanced by another source of spherical aberration, leading to a shift in the position of the maximum efficiency in the Z direction. For a 1.1 microm polystyrene bead we could achieve the maximal efficiency at a depth of 70 microm, whereas the trap was stable up to a depth of 100 microm.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.31.000766