Optically Addressed Droplet-Based Protein Assay

We demonstrate a new method for performing protein assays with very small volumes (∼1.7 μL to 14 pL). Using a laser to modify local surface energy, we manipulate, fuse, and mix droplets containing horseradish peroxidase and its substrates. A detection limit of ∼30 attomoles of reacting enzyme was me...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2005-04, Vol.127 (16), p.5736-5737
Main Authors: Kotz, Kenneth T, Gu, Yu, Faris, Gregory W
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Benzothiazoles
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Horseradish Peroxidase - analysis
Horseradish Peroxidase - chemistry
Hydrogen Peroxide - chemistry
Indicators and Reagents - chemistry
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Optics and Photonics - instrumentation
Oxidation-Reduction
Polystyrenes - chemistry
Proteins
Proteins - analysis
Proteins - chemistry
Sulfonic Acids - chemistry
Surface Tension
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Summary:We demonstrate a new method for performing protein assays with very small volumes (∼1.7 μL to 14 pL). Using a laser to modify local surface energy, we manipulate, fuse, and mix droplets containing horseradish peroxidase and its substrates. A detection limit of ∼30 attomoles of reacting enzyme was measured by optical absorption. We discuss the possibility of extending this lower limit to zeptomoles of enzyme.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja050249m