High salinity reduces the content of a highly abundant 23-kDa protein of the mangrove Bruguiera parviflora

• Published in: Planta 2005-04, Vol.221 (1), p.135-140
• Main Authors: Parida, Asish Kumar, Mittra, Bhabatosh, Das, Anath Bandhu, Das, Taposh Kumar, Mohanty, Prasanna
• Format: Article
• Language: English
• Subjects: Antibodies; Biological and medical sciences; Blotting, Western; Cell biochemistry; Cell physiology; Chloroplasts; Chloroplasts - chemistry; Chloroplasts - metabolism; Cytosol; Electrophoresis, Polyacrylamide Gel; Enzymes; Fundamental and applied biological sciences. Psychology; Gels; Leaves; Molecular Weight; Osmolar Concentration; Plant Leaves - drug effects; Plant Leaves - metabolism; Plant physiology and development; Plant Proteins - isolation & purification; Plant Proteins - metabolism; Plants; Proteins; Rhizophoraceae - drug effects; Rhizophoraceae - metabolism; Salts; Seedlings; Sodium chloride; Sodium Chloride - pharmacology; Table salt
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-004-1415-2

A significant decrease in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 23 kDa and pI = 6.5, was observed in leaves of NaCl-treated Bruguiera parviflora (Roxb.) Wt. & Arn. ex Griff. seedlings. This particular salt-sensitive protein, designated as SSP-23, almost disappeared after 45 days of treatment in 400 mM NaCl as compared to untreated seedlings (0 mM NaCl) where the presence of the protein was significant. A polyclonal antibody raised against the 23-kDa protein was used to determine the subcellular localization of this protein in leaves by cross-reaction with proteins from isolated chloroplasts, mitochondria, peroxisomes and cytosol fractions on Western blots. SSP-23 was confirmed to be localized in the cytosol by immunoblotting. The disappearance of SSP-23 as a result of high NaCl treatment suggests that this protein is salt-sensitive and has a possible role in salt adaptation.