Enhanced mucosal immunogenicity of prion protein following fusion with B subunit of Escherichia coli heat-labile enterotoxin

Mucosal vaccine against prion protein (PrP), a major component of prions, is urgently awaited since the oral transmission of prions from cattle to humans is highly suspected. In the present study, we produced recombinant bovine and mouse PrPs fused with or without the B subunit of Escherichia coli h...

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Bibliographic Details
Published in:Vaccine 2006-04, Vol.24 (15), p.2815-2823
Main Authors: Yamanaka, Hitoki, Ishibashi, Daisuke, Yamaguchi, Naohiro, Yoshikawa, Daisuke, Nakamura, Risa, Okimura, Nobuhiko, Arakawa, Takeshi, Tsuji, Takao, Katamine, Shigeru, Sakaguchi, Suehiro
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Antibodies - blood
Applied microbiology
Bacterial Toxins - genetics
Bacterial Toxins - immunology
Bacteriology
Biological and medical sciences
Enterotoxins - genetics
Enterotoxins - immunology
Escherichia coli
Escherichia coli Proteins - genetics
Escherichia coli Proteins - immunology
Female
Fundamental and applied biological sciences. Psychology
Heat-labile enterotoxin
Immunity, Mucosal
Immunoglobulin A - blood
Immunoglobulin G - blood
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microbiology
Miscellaneous
Mucosal vaccine
Prion
Prions - genetics
Prions - immunology
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Mucosal vaccine against prion protein (PrP), a major component of prions, is urgently awaited since the oral transmission of prions from cattle to humans is highly suspected. In the present study, we produced recombinant bovine and mouse PrPs fused with or without the B subunit of Escherichia coli heat-labile enterotoxin (LTB) and intranasally immunized mice with these fused proteins. Fusion with LTB markedly enhanced the mucosal immunogenicity of bovine PrP, producing a marked increase in specific IgG and IgA titer in serum. Mouse PrP also showed slightly increased immunogenicity following fusion with LTB. These results demonstrate that LTB-fused PrPs might be potential candidates for protective mucosal prion vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2005.12.054