Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander

When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP=15-30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 complementary...

Full description

Saved in:
Bibliographic Details
Published in:Development genes and evolution 2006-04, Vol.216 (4), p.224-228
Main Authors: Sakata, Natsuko, Miyazaki, Kinuko, Wakahara, Masami
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Animals
Aromatase - genetics
Body Temperature - genetics
Disorders of Sex Development
Dmrt-1
DNA, Complementary
Down-Regulation
Female
Freshwater
Gene Expression Regulation, Enzymologic
Genes
Hynobius retardatus
Male
Molecular Sequence Data
Ovary - enzymology
P450arom
Protein Structure, Tertiary
salamanders and newts
Sequence Homology, Amino Acid
Sex Differentiation - genetics
Sex reversal
sexual development
Temperature
Testis - enzymology
Up-Regulation
Urodela - genetics
Urodela - growth & development
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c490t-9ca19a4d236c435e88077c860244235f190c5be88afdfb3bf81192c82e267b2a3
cites cdi_FETCH-LOGICAL-c490t-9ca19a4d236c435e88077c860244235f190c5be88afdfb3bf81192c82e267b2a3
container_end_page 228
container_issue 4
container_start_page 224
container_title Development genes and evolution
container_volume 216
creator Sakata, Natsuko
Miyazaki, Kinuko
Wakahara, Masami
description When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP=15-30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 complementary DNAs were isolated and their expression patterns were analyzed by competitive and conventional reverse transcriptase-polymerase chain reaction. While the P450arom gene was expressed predominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae were reared at the female-producing temperature (28°C) during the TSP, a strong expression of the P450arom gene and a complete suppression of the Dmrt-1 gene were induced in all experimental larvae. Up-regulation of the P450arom gene and down-regulation of the Dmrt-1 gene even in genetic males constitute a part of the molecular biological cascade for the temperature-dependent sex reversal from genetic males to phenotypic females in this salamander.
doi_str_mv 10.1007/s00427-005-0053-4
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67765850</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67765850</sourcerecordid><originalsourceid>FETCH-LOGICAL-c490t-9ca19a4d236c435e88077c860244235f190c5be88afdfb3bf81192c82e267b2a3</originalsourceid><addsrcrecordid>eNqFkU-L1TAUxYsoznP0A7jR4MJd9OZPm3QpM_6DAQV9MLuQ19686dAmNUnV-TZ-VFP6QJiNi0vg5HcO93Kq6jmDNwxAvU0AkisKUK8jqHxQ7ZgUnIJk1w-rHbSypa2U12fVk5RuARhvRf24OmNNDQ0o2FV_9jONeFxGm4fgSXDkq6zBxjAR63vSh1_-3v_lFDNl5IgeExk8yTdIMk4zRpuXiLTHGX2PPpOEv0nEnxiTHYlbI1dTHjoy2bGYcyDzDfqQ7-aiOdzUEmlJcdipLIDxafXI2THhs9N7Xu0_vP9-8Yleffn4-eLdFe1kC5m2nWWtlT0XTSdFjVqDUp1ugEvJRe1YC119KLJ1vTuIg9OMtbzTHHmjDtyK8-r1ljvH8GPBlM00pA7H0XoMSzKNUk2ta_gvWGKV0pIV8NU98DYs0ZcjTFlOMi10UyC2QV0MKUV0Zo7DZOOdYWDWks1WsikFryOMLJ4Xp-DlMGH_z3FqtQAvN8DZYOwxDsnsv3FgAkA3UrVK_AWtqKyU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>807418386</pqid></control><display><type>article</type><title>Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander</title><source>Springer Link</source><creator>Sakata, Natsuko ; Miyazaki, Kinuko ; Wakahara, Masami</creator><creatorcontrib>Sakata, Natsuko ; Miyazaki, Kinuko ; Wakahara, Masami</creatorcontrib><description>When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP=15-30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 complementary DNAs were isolated and their expression patterns were analyzed by competitive and conventional reverse transcriptase-polymerase chain reaction. While the P450arom gene was expressed predominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae were reared at the female-producing temperature (28°C) during the TSP, a strong expression of the P450arom gene and a complete suppression of the Dmrt-1 gene were induced in all experimental larvae. Up-regulation of the P450arom gene and down-regulation of the Dmrt-1 gene even in genetic males constitute a part of the molecular biological cascade for the temperature-dependent sex reversal from genetic males to phenotypic females in this salamander.</description><identifier>ISSN: 0949-944X</identifier><identifier>EISSN: 1432-041X</identifier><identifier>DOI: 10.1007/s00427-005-0053-4</identifier><identifier>PMID: 16506070</identifier><language>eng</language><publisher>Germany: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Aromatase - genetics ; Body Temperature - genetics ; Disorders of Sex Development ; Dmrt-1 ; DNA, Complementary ; Down-Regulation ; Female ; Freshwater ; Gene Expression Regulation, Enzymologic ; Genes ; Hynobius retardatus ; Male ; Molecular Sequence Data ; Ovary - enzymology ; P450arom ; Protein Structure, Tertiary ; salamanders and newts ; Sequence Homology, Amino Acid ; Sex Differentiation - genetics ; Sex reversal ; sexual development ; Temperature ; Testis - enzymology ; Up-Regulation ; Urodela - genetics ; Urodela - growth &amp; development</subject><ispartof>Development genes and evolution, 2006-04, Vol.216 (4), p.224-228</ispartof><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-9ca19a4d236c435e88077c860244235f190c5be88afdfb3bf81192c82e267b2a3</citedby><cites>FETCH-LOGICAL-c490t-9ca19a4d236c435e88077c860244235f190c5be88afdfb3bf81192c82e267b2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16506070$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakata, Natsuko</creatorcontrib><creatorcontrib>Miyazaki, Kinuko</creatorcontrib><creatorcontrib>Wakahara, Masami</creatorcontrib><title>Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander</title><title>Development genes and evolution</title><addtitle>Dev Genes Evol</addtitle><description>When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP=15-30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 complementary DNAs were isolated and their expression patterns were analyzed by competitive and conventional reverse transcriptase-polymerase chain reaction. While the P450arom gene was expressed predominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae were reared at the female-producing temperature (28°C) during the TSP, a strong expression of the P450arom gene and a complete suppression of the Dmrt-1 gene were induced in all experimental larvae. Up-regulation of the P450arom gene and down-regulation of the Dmrt-1 gene even in genetic males constitute a part of the molecular biological cascade for the temperature-dependent sex reversal from genetic males to phenotypic females in this salamander.</description><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Aromatase - genetics</subject><subject>Body Temperature - genetics</subject><subject>Disorders of Sex Development</subject><subject>Dmrt-1</subject><subject>DNA, Complementary</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Freshwater</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Genes</subject><subject>Hynobius retardatus</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Ovary - enzymology</subject><subject>P450arom</subject><subject>Protein Structure, Tertiary</subject><subject>salamanders and newts</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sex Differentiation - genetics</subject><subject>Sex reversal</subject><subject>sexual development</subject><subject>Temperature</subject><subject>Testis - enzymology</subject><subject>Up-Regulation</subject><subject>Urodela - genetics</subject><subject>Urodela - growth &amp; development</subject><issn>0949-944X</issn><issn>1432-041X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkU-L1TAUxYsoznP0A7jR4MJd9OZPm3QpM_6DAQV9MLuQ19686dAmNUnV-TZ-VFP6QJiNi0vg5HcO93Kq6jmDNwxAvU0AkisKUK8jqHxQ7ZgUnIJk1w-rHbSypa2U12fVk5RuARhvRf24OmNNDQ0o2FV_9jONeFxGm4fgSXDkq6zBxjAR63vSh1_-3v_lFDNl5IgeExk8yTdIMk4zRpuXiLTHGX2PPpOEv0nEnxiTHYlbI1dTHjoy2bGYcyDzDfqQ7-aiOdzUEmlJcdipLIDxafXI2THhs9N7Xu0_vP9-8Yleffn4-eLdFe1kC5m2nWWtlT0XTSdFjVqDUp1ugEvJRe1YC119KLJ1vTuIg9OMtbzTHHmjDtyK8-r1ljvH8GPBlM00pA7H0XoMSzKNUk2ta_gvWGKV0pIV8NU98DYs0ZcjTFlOMi10UyC2QV0MKUV0Zo7DZOOdYWDWks1WsikFryOMLJ4Xp-DlMGH_z3FqtQAvN8DZYOwxDsnsv3FgAkA3UrVK_AWtqKyU</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Sakata, Natsuko</creator><creator>Miyazaki, Kinuko</creator><creator>Wakahara, Masami</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander</title><author>Sakata, Natsuko ; Miyazaki, Kinuko ; Wakahara, Masami</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-9ca19a4d236c435e88077c860244235f190c5be88afdfb3bf81192c82e267b2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Motifs</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Aromatase - genetics</topic><topic>Body Temperature - genetics</topic><topic>Disorders of Sex Development</topic><topic>Dmrt-1</topic><topic>DNA, Complementary</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Freshwater</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Genes</topic><topic>Hynobius retardatus</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Ovary - enzymology</topic><topic>P450arom</topic><topic>Protein Structure, Tertiary</topic><topic>salamanders and newts</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sex Differentiation - genetics</topic><topic>Sex reversal</topic><topic>sexual development</topic><topic>Temperature</topic><topic>Testis - enzymology</topic><topic>Up-Regulation</topic><topic>Urodela - genetics</topic><topic>Urodela - growth &amp; development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakata, Natsuko</creatorcontrib><creatorcontrib>Miyazaki, Kinuko</creatorcontrib><creatorcontrib>Wakahara, Masami</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest_Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Development genes and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakata, Natsuko</au><au>Miyazaki, Kinuko</au><au>Wakahara, Masami</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander</atitle><jtitle>Development genes and evolution</jtitle><addtitle>Dev Genes Evol</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>216</volume><issue>4</issue><spage>224</spage><epage>228</epage><pages>224-228</pages><issn>0949-944X</issn><eissn>1432-041X</eissn><abstract>When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP=15-30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 complementary DNAs were isolated and their expression patterns were analyzed by competitive and conventional reverse transcriptase-polymerase chain reaction. While the P450arom gene was expressed predominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae were reared at the female-producing temperature (28°C) during the TSP, a strong expression of the P450arom gene and a complete suppression of the Dmrt-1 gene were induced in all experimental larvae. Up-regulation of the P450arom gene and down-regulation of the Dmrt-1 gene even in genetic males constitute a part of the molecular biological cascade for the temperature-dependent sex reversal from genetic males to phenotypic females in this salamander.</abstract><cop>Germany</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>16506070</pmid><doi>10.1007/s00427-005-0053-4</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0949-944X
ispartof Development genes and evolution, 2006-04, Vol.216 (4), p.224-228
issn 0949-944X
1432-041X
language eng
recordid cdi_proquest_miscellaneous_67765850
source Springer Link
subjects Amino Acid Motifs
Amino Acid Sequence
Animals
Aromatase - genetics
Body Temperature - genetics
Disorders of Sex Development
Dmrt-1
DNA, Complementary
Down-Regulation
Female
Freshwater
Gene Expression Regulation, Enzymologic
Genes
Hynobius retardatus
Male
Molecular Sequence Data
Ovary - enzymology
P450arom
Protein Structure, Tertiary
salamanders and newts
Sequence Homology, Amino Acid
Sex Differentiation - genetics
Sex reversal
sexual development
Temperature
Testis - enzymology
Up-Regulation
Urodela - genetics
Urodela - growth & development
title Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperature-dependent sex reversal from genetic males to phenotypic females in a salamander
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T04%3A45%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Up-regulation%20of%20P450arom%20and%20down-regulation%20of%20Dmrt-1%20genes%20in%20the%20temperature-dependent%20sex%20reversal%20from%20genetic%20males%20to%20phenotypic%20females%20in%20a%20salamander&rft.jtitle=Development%20genes%20and%20evolution&rft.au=Sakata,%20Natsuko&rft.date=2006-04-01&rft.volume=216&rft.issue=4&rft.spage=224&rft.epage=228&rft.pages=224-228&rft.issn=0949-944X&rft.eissn=1432-041X&rft_id=info:doi/10.1007/s00427-005-0053-4&rft_dat=%3Cproquest_cross%3E67765850%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c490t-9ca19a4d236c435e88077c860244235f190c5be88afdfb3bf81192c82e267b2a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=807418386&rft_id=info:pmid/16506070&rfr_iscdi=true