Simultaneous determination of nine saponins from Panax notoginseng using HPLC and pressurized liquid extraction

A HPLC and pressurized liquid extraction (PLE) method was developed for simultaneous determination of nine saponins, including notoginsenoside R1, ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3 and Rd in Panax notoginseng. The analysis was performed on C 18 column with water–acetonitrile gradient elutio...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2006-04, Vol.41 (1), p.274-279
Main Authors: Wan, J.B., Lai, C.M., Li, S.P., Lee, M.Y., Kong, L.Y., Wang, Y.T.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Calibration
Chemistry, Pharmaceutical - methods
Chromatography
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Ginsenoside
Ginsenosides - analysis
Ginsenosides - pharmacology
Mass Spectrometry
Medical sciences
Models, Chemical
Notoginsenoside
Panax - metabolism
Panax notoginseng
Pharmacology. Drug treatments
Plant Extracts - analysis
Plant Extracts - chemistry
Pressurized liquid extraction
Saponins - analysis
Saponins - chemistry
Spectrometry, Mass, Electrospray Ionization - methods
Technology, Pharmaceutical - methods
Time Factors
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Summary:A HPLC and pressurized liquid extraction (PLE) method was developed for simultaneous determination of nine saponins, including notoginsenoside R1, ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3 and Rd in Panax notoginseng. The analysis was performed on C 18 column with water–acetonitrile gradient elution and the investigated saponins were authenticated by comparing retention time and mass spectra with their reference compounds. Several methods including PLE, ultrasonication, soxhlet extraction and immersion were used for sample preparation and their extraction efficiency was compared. The results showed that PLE has the highest extraction efficiency and repeatability, which would be valuable on standardization of sample preparation for quality control of Chinese medicines. The developed HPLC and PLE is an effective approach for simultaneously quantitative determination of sapoinins in P. notoginseng, which could be used for quality control of P. notoginseng and its preparations.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2005.10.023