Emergence of nuclear heparanase induces differentiation of human mammary cancer cells

The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, ce...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-05, Vol.331 (1), p.175-180
Main Authors: Nobuhisa, Tetsuji, Naomoto, Yoshio, Takaoka, Munenori, Tabuchi, Yoko, Ookawa, Keizou, Kitamoto, Dai, Gunduz, Esra, Gunduz, Mehmet, Nagatsuka, Hitoshi, Haisa, Minoru, Matsuoka, Junji, Nakajima, Motowo, Tanaka, Noriaki
Format: Article
Language:English
Subjects:
Breast Neoplasms - enzymology
Breast Neoplasms - pathology
Breast Neoplasms - ultrastructure
Cell Differentiation
Cell Line, Tumor
Cell Nucleus - enzymology
Differentiation
Epithelial cancer
Female
Genetic Vectors
Glucuronidase - analysis
Glucuronidase - genetics
Glucuronidase - metabolism
Heparanase
Humans
Lipids - analysis
MCF-7
Nucleus
Transfection
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Summary:The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, cell death. There are spatially distinct compartments dedicated to each of these events. Recently we reported that heparanase is expressed in nucleus as well as in the cytoplasm and that nuclear heparanase seems to be related to cell differentiation. In this study, we investigated the role of nuclear heparanase in differentiation by transducing human mammary epithelial cancer cells with heparanase which was delivered specifically into nucleus. We observed that expression of nuclear heparanase allowed the cells to differentiate with the appearance of lipid droplets. This finding supports the idea that heparanase plays a novel role in epithelial cell differentiation apart from its known enzymatic function.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.03.129