Broadband reflectance measurements of light penetration, blood oxygenation, hemoglobin concentration, and drug concentration in human intraperitoneal tissues before and after photodynamic therapy

• Published in: Journal of Biomedical Optics 2005-01, Vol.10 (1), p.014004-0140013
• Main Authors: Wang, Hsing-Wen, Zhu, Timothy C, Putt, Mary E, Solonenko, Michael, Metz, James, Dimofte, Andreea, Miles, Jeremy, Fraker, Douglas L, Glatstein, Eli, Hahn, Stephen M, Yodh, Arjun G
• Format: Article
• Language: English
• Subjects: Algorithms; Chemotherapy, Adjuvant; diffuse reflectance spectroscopy; Dihematoporphyrin Ether - pharmacokinetics; Dihematoporphyrin Ether - therapeutic use; Hematoporphyrin Photoradiation; Hemoglobins - metabolism; Humans; Intestines - metabolism; intraperitoneal optical properties; Light; Liver - metabolism; Osmolar Concentration; Oxygen - blood; peritoneal carcinomatosis; Peritoneal Neoplasms - drug therapy; Peritoneal Neoplasms - surgery; Peritoneum - metabolism; photodynamic therapy; photodynamic therapy dosimetry; physiological properties; Scattering, Radiation; Skin - metabolism; Tissue Distribution
• ISSN: 1083-3668; 1560-2281
• DOI: 10.1117/1.1854679

We evaluate Photofrin-mediated photodynamic therapy (PDT) in a phase 2 clinical trial as an adjuvant to surgery to treat peritoneal carcinomatosis. We extract tissue optical [reduced scattering absorption and attenuation coefficients and physiological [blood oxygen saturation total hemoglobin concentration (THC), and photosensitizer concentration properties in 12 patients using a diffuse reflectance instrument and algorithms based on the diffusion equation. Before PDT, in normal intraperitoneal tissues and THC ranged between 32 to 100 and 19 to 263 M, respectively; corresponding data from tumor tissues ranged between 11 to 44 and 61 to 224 M. Tumor is significantly lower than oxygenation of normal intraperitoneal tissues in the same patients. The mean (±standard error of mean) penetration depth ( ) in millimeters at 630 nm is 4.8(±0.6) for small bowel, 5.2 (±0.67) for large bowel, 3.39(±0.29) for peritoneum, 5.19(±1.4) for skin, 1.0(±0.1) for liver, and 3.02(±0.66) for tumor. in micromolars is 4.9(±2.3) for small bowel, 4.8(±2.3) for large bowel, 3.0 (±1.0) for peritoneum, 2.5(±0.9) for skin, and 7.4(±2.8) for tumor. In all tissues examined, mean tends to decrease after PDT, perhaps due to photobleaching. These results provide benchmark tissue optical property data, and demonstrate the feasibility of measurements during clinical PDT treatments. ©