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Microglial expression of chemokine receptor CCR5 during rat forebrain development and after perinatal hypoxia–ischemia

The chemokine macrophage inflammatory protein 1α (CCL3) is expressed by immune cells in the normal and injured perinatal brain. To determine whether the chemokine receptor CCR5 is a relevant target for CCL3 in the brain, we used RT-PCR and immunocytochemistry to assess changes in CCR5 expression and...

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Published in:	Journal of neuroimmunology 2006-04, Vol.173 (1), p.155-165
Main Authors:	Cowell, Rita M., Xu, Haiyan, Parent, Jack M., Silverstein, Faye S.
Format:	Article
Language:	English
Subjects:	Animals Animals, Newborn CCL3 Dentate gyrus Embryonic rat Hypoxia-Ischemia, Brain - physiopathology Immunohistochemistry Microglia Microglia - metabolism Microscopy, Confocal Neonatal stroke Prosencephalon - growth & development Prosencephalon - metabolism Prosencephalon - pathology Rats Receptors, CCR5 - biosynthesis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis
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creator	Cowell, Rita M. Xu, Haiyan Parent, Jack M. Silverstein, Faye S.
description	The chemokine macrophage inflammatory protein 1α (CCL3) is expressed by immune cells in the normal and injured perinatal brain. To determine whether the chemokine receptor CCR5 is a relevant target for CCL3 in the brain, we used RT-PCR and immunocytochemistry to assess changes in CCR5 expression and localization in developing normal and injured rat forebrain. CCR5 protein was expressed predominately by resting and activated microglia until 2 weeks of age. Neonatal hypoxia–ischemia increased CCR5 mRNA expression while causing CCR5 internalization, indicating receptor activation. These data implicate CCR5 in microglial recruitment and activation during brain development and after neonatal brain injury.
doi_str_mv	10.1016/j.jneuroim.2006.01.005
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subjects	Animals Animals, Newborn CCL3 Dentate gyrus Embryonic rat Hypoxia-Ischemia, Brain - physiopathology Immunohistochemistry Microglia Microglia - metabolism Microscopy, Confocal Neonatal stroke Prosencephalon - growth & development Prosencephalon - metabolism Prosencephalon - pathology Rats Receptors, CCR5 - biosynthesis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis
title	Microglial expression of chemokine receptor CCR5 during rat forebrain development and after perinatal hypoxia–ischemia
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