Marking cell lineages in living tissues

We have generated a novel genetic system to visualize cell lineages in living tissues at high resolution. Heat shock was used to trigger the excision of a specific transposon and activation of a fluorescent marker gene. A histone-YFP marker was used to allow identification of cell lineages and easy...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2005-05, Vol.42 (3), p.444-453
Main Authors: Kurup, S, Runions, J, Kohler, U, Laplaze, L, Haseloff, J
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - cytology
Arabidopsis - growth & development
Arabidopsis thaliana
Biological and medical sciences
Botany
Botany - methods
Cell Differentiation
Cell Division
Cell kinetics
cell lineage
Cell Lineage - physiology
cell lineages
Cell physiology
Cells
Flowers & plants
Fundamental and applied biological sciences. Psychology
Genes, Reporter
green fluorescent protein
Heat
heat shock
heat stress
histones
Hot Temperature
laser induction
Lasers
live cell
Luminescent Proteins - metabolism
plant genetics
Plant physiology and development
Plant Roots - cytology
Plants, Genetically Modified
Recombinant Fusion Proteins - metabolism
root development
transposons
YFP
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Summary:We have generated a novel genetic system to visualize cell lineages in living tissues at high resolution. Heat shock was used to trigger the excision of a specific transposon and activation of a fluorescent marker gene. A histone-YFP marker was used to allow identification of cell lineages and easy counting of cells. Constitutive expression of a green fluorescent membrane protein was used to provide a precise outline of all surrounding cells. Marked lineages can be induced from specific cells within the organism by targeted laser irradiation, and the fate of the marked cells can be followed non-invasively. We have used the system to map cell lineages originating from the initials of primary and lateral roots in Arabidopsis. The lineage marking technique enabled us to measure the differential contribution of primary root pericycle cell files to developing lateral root primordia. The majority of cells in an emerging lateral root primordium derive from the central file of pericycle founder cells while off-centre founder cells contribute only a minor proliferation of tissue near the base of the root. The system shows great promise for the detailed study of cell division during morphogenesis.
ISSN:0960-7412
1365-313X
DOI:10.1111/j.1365-313X.2005.02386.x