Fourier transform infrared imaging spectroscopy analysis of collagenase-induced cartilage degradation

Collagenase treatment of cartilage serves as an model of the pathological collagen degradation that occurs in the disease osteoarthritis (OA). Fourier transform infrared imaging spectroscopic (FT-IRIS) analysis of collagenase-treated cartilage is performed to elucidate the molecular origin of the sp...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2005-01, Vol.10 (1), p.014015-014016
Main Authors: West, P. A, Torzilli, P. A, Chen, C, Lin, P, Camacho, N. P
Format: Article
Language:English
Subjects:
Animals
Cartilage, Articular - drug effects
Cartilage, Articular - pathology
Cattle
collagen
Collagen - ultrastructure
collagenase
Collagenases - pharmacology
Fluorescent Antibody Technique
Humans
infrared fiber optic probe
infrared imaging
Knee Joint
osteoarthritis
Osteoarthritis - pathology
Spectroscopy, Fourier Transform Infrared
Staining and Labeling
Time Factors
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Summary:Collagenase treatment of cartilage serves as an model of the pathological collagen degradation that occurs in the disease osteoarthritis (OA). Fourier transform infrared imaging spectroscopic (FT-IRIS) analysis of collagenase-treated cartilage is performed to elucidate the molecular origin of the spectral changes previously found at the articular surface of human OA cartilage. Bovine cartilage explants are treated with 0.1 collagenase for 0, 15, or 30 min. collagen cleavage is assessed using immunofluorescent staining with an antibody specific for broken type II collagen. The FT-IRIS analysis of the control and treated specimens mirrors the differences previously found between normal and OA cartilage using an infrared fiber optic probe (IFOP). With collagenase treatment, the amide II/1338 cm area ratio increases while the 1238 cm /1227 cm peak ratio decreases. In addition, polarized FT-IRIS demonstrates a more random orientation of the collagen fibrils that correlate spatially with the immunofluorescent-determined regions of broken type II collagen. We can therefore conclude that the spectral changes observed in the collagenase-treated cartilage, and similarly in OA cartilage, arise from changes in collagen structure. These findings support the use of mid-infrared spectral analysis, in particular the minimally invasive IFOP, as potential techniques for the diagnosis and management of degenerative joint diseases such as osteoarthritis. ©
ISSN:1083-3668
1560-2281
DOI:10.1117/1.1854131